一株琼胶酶产生菌的分离及产酶培养基优化  

Isolation of an agarase-producing bacterium from the soil and optimization of cultural medium

作　　者：陈虹[1] 张建芬[1] 柯薇[1] 

机构地区：[1]浙江树人大学生物与环境工程学院,杭州310015

出　　处：《食品科技》2014年第4期10-14,共5页Food Science and Technology

基　　金：浙江树人大学科研项目(2010A31003)

摘　　要：用高氏Ⅰ号平板培养基从土壤中分离到一株琼胶酶产生菌TQ8,依据形态学特征和16S rDNA序列分析,鉴定其为一株不动杆菌(Acinetobacter sp.)。通过单因素实验和正交实验优化了该菌株产琼胶酶的培养基主要组成及其pH值,在产酶培养基组成为葡萄糖10 g/L、琼脂2 g/L、复合氮源[蛋白胨:(NH4)2SO4:KNO3=3:1:1]7 g/L、酵母浸出粉5 g/L、NaCl 5 g/L、MgSO4·7H2O 0.3 g/L,pH为7.0时,TQ8菌株产酶活力达到83.5 U/mL,较优化前的21.7 U/mL提高了2.85倍。An agarase-producing bacterium designated as the strain TQ8 was isolated from the soil by using the Gause＇s I agar plate. Based on morphological characteristics and 16S rDNA sequence comparison, the strain TQ8 was identified as a member of the Acinetobacter. For improving agarase productivity of the strain, the composition of the culture medium and pH were optimized by single-factor and orthogonal experiment. The results showed that the optimal medium was composed of glucose 10 g/L, agar 2 g/L, nitrogen source （peptone：（NH4）2SO4：KNO3=3：l：l） 7 g/L, yeast extract 5 g/L, NaCI 5 g/L and MgSO4.7H20 0.3 g/L, and the favorable initial pH was 7.0. Under the optimized culture medium, the agarase activity of TQ8 could reach 83.5 U/mL, which was 2.85 times higher than 21.7 U/m L of that under the initial one.

关 键 词：琼胶酶 不动杆菌 分离与鉴定 培养基优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]