Effector cells derived from naive T cells used in tumor immunotherapy of mice bearing B16 melanoma  

作　　者：Wen Ming Xu Weili Ren Lili Gao Fei Cui Naipeng Wen Junye Li Xinjiang Lin Lin Ma Zhenfeng Chen Baoping Cai Jianhui 

机构地区：[1]Department of Surgery, Hebei Medical University, Shijiazhuang,Hebei 050017, China [2]Department of Surgical Oncology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, China [3]Department of Pediatric Surgery, the Second Hospital of Hebei Medical University,Shijiazhuang, Hebei 050000, China [4]Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, China [5]Teaching & Research Section, Hebei Medical University,Shijiazhuang, Hebei 050017 [6]Department of Surgery, Department of Oncology & Immunotherapy, Hebei General Hospital, Shijiazhuang,Hebei 050051, China [7]Department of Surgery, the Second Hospital of Hebei Medical University,Shijiazhuang, Hebei 050000, China

出　　处：《Chinese Medical Journal》2014年第7期1328-1333,共6页中华医学杂志（英文版）

摘　　要：Background Adoptive cell transfer （ACT） immunotherapy has been used clinically for years to treat malignancies.Improving the killing efficiency of effector cells,such as tumor-specific cytotoxic T lymphocytes （CTLs）,is an important component for enhancing the clinical response of cancer immunotherapy.Hence,we explored a novel method for preparing cancer-specific CTLs using naive T lymphocytes.Methods C57BL/6 mice bearing B16 melanoma tumors were pretreated with cyclophosphamide （CTX） by peritoneal injection.The immunosuppressive influence of CTX on tumor regression and the tumor microenvironment was assessed.Naive T cells and T cell pools were isolated via negative selection using immunomagnetic beads.The proliferative potential and cytokine production of different T cell subpopulations were evaluated in vitro.Tumor-specific CTLs derived from naive T cells （naive CD4＋ T cells：naive CD8＋ T cells=2：1） and pooled T cells were generated in vitro,respectively.B16 melanoma-bearing C57BL/6 mice were pretreated with CTX,followed by ACT immunotherapy using dendritic cell-induced CTLs.The homing abilities of the effector cells and interleukin-2 （IL-2）,interferon-y,granzyme B,and perforin mRNA levels in tumor tissues were evaluated,and the change in tumor volume was measured.Results Mice receiving CTX peritoneal pretreatment injections did not display tumor regression compared with control mice.However,a significant downregulation of splenic Tregs and tumor growth factor-β1 （TGF-β1） and interleukin-10 （IL-10） serum levels was observed （P ＜0.05）.Naive T cells showed a stronger proliferative capacity and elevated cytokine production than did pooled T cells （P ＜0.05）.In addition,effector cells generated from naive T cells displayed more potent antitumor activity in vivo than those derived from pooled T cells （P ＜0.05）.Conclusion Effector cells derived from the naive T cells possess a stronger proliferative potential,homing capacity,and enhanced cytokine production,which lBackground Adoptive cell transfer （ACT） immunotherapy has been used clinically for years to treat malignancies.Improving the killing efficiency of effector cells,such as tumor-specific cytotoxic T lymphocytes （CTLs）,is an important component for enhancing the clinical response of cancer immunotherapy.Hence,we explored a novel method for preparing cancer-specific CTLs using naive T lymphocytes.Methods C57BL/6 mice bearing B16 melanoma tumors were pretreated with cyclophosphamide （CTX） by peritoneal injection.The immunosuppressive influence of CTX on tumor regression and the tumor microenvironment was assessed.Naive T cells and T cell pools were isolated via negative selection using immunomagnetic beads.The proliferative potential and cytokine production of different T cell subpopulations were evaluated in vitro.Tumor-specific CTLs derived from naive T cells （naive CD4＋ T cells：naive CD8＋ T cells=2：1） and pooled T cells were generated in vitro,respectively.B16 melanoma-bearing C57BL/6 mice were pretreated with CTX,followed by ACT immunotherapy using dendritic cell-induced CTLs.The homing abilities of the effector cells and interleukin-2 （IL-2）,interferon-y,granzyme B,and perforin mRNA levels in tumor tissues were evaluated,and the change in tumor volume was measured.Results Mice receiving CTX peritoneal pretreatment injections did not display tumor regression compared with control mice.However,a significant downregulation of splenic Tregs and tumor growth factor-β1 （TGF-β1） and interleukin-10 （IL-10） serum levels was observed （P ＜0.05）.Naive T cells showed a stronger proliferative capacity and elevated cytokine production than did pooled T cells （P ＜0.05）.In addition,effector cells generated from naive T cells displayed more potent antitumor activity in vivo than those derived from pooled T cells （P ＜0.05）.Conclusion Effector cells derived from the naive T cells possess a stronger proliferative potential,homing capacity,and enhanced cytokine production,which l

关 键 词：naive T cell pooled T cells cytotoxic T lymphocyte MELANOMA IMMUNOTHERAPY adoptive transfer 

分 类 号：R739.5[医药卫生—肿瘤]