胶体金免疫层析法快速定量检测猪肝中喹乙醇残留  

Development of colloidal-gold Immunochromatographic strip for rapid and quantitative detection of olaquindox in pig liver

作　　者：霍如林 朱爱荣[1] 张林[1] 高峰[1] 周光宏[1] 

机构地区：[1]南京农业大学动物科技学院/江苏省动物源食品生产与安全保障重点实验室,江苏南京210095

出　　处：《食品工业科技》2014年第9期299-302,共4页Science and Technology of Food Industry

基　　金："十二五"国家科技支撑计划课题(2012BAD28B03);江苏省农业三新工程项目(SX(2011)146)

摘　　要：本文研究了胶体金免疫层析试纸条快速定量检测猪肝中喹乙醇残留的方法。利用胶体金免疫层析技术，制备了喹乙醇胶体金试纸条，检测了其特异性，并建立了T／C比值法进行定量检测猪肝中喹乙醇的残留。制备的试纸条可以在15min内完成定性和定量检测，与卡巴氧、乙酰甲喹和3-甲基-喹嚷啉-2-羟酸几乎无交叉反应。当肝中喹乙醇质量浓度在1～200ng／mL范围内，试纸条有较好的线性关系，最低检测限为6．83ng／mL。阴性加标实验结果表明，喹乙醇浓度在25、50、100ng/mL时，试纸条的回收率在90．9％-105．0％之间。The aim of the present study was to develop colloidal gold immunochromatography assays for rapid and quantitative detection of olaquindox（QLA）residues in pig liver.Prepared the colloidal gold immunochromatography test strips and evaluated the specificity,a quantitative method based on T/C ratio was developed to detect OLA residue in pig liver.Preparation of the test strip could be accomplished qualitatively as well as quantitatively detection in 15min, and there was little cross- reactivity detected with carbadox, mequindox and 3 - methyl- quinoxaline-2-hydroxy acids.When the pig liver had OLA concentration at a range of 1 to 200ng/mL,the test strip showed a higher linear relationship,and the limit of detection（LQD） of immunochromatographic strip for QLA was 6.83ng/mL.For negative liver samples,the recoveries rate was 90.9%-105.0% at 25,50 and lOOna/mL.

关 键 词：胶体金免疫层析 试纸条 喹乙醇 定量检测 猪肝 

分 类 号：TS201.6[轻工技术与工程—食品科学]