牛瘤胃细菌产木聚糖酶菌株的筛选及酶学性质研究  

Screening of Xylanase Producing Bacteria from Bovine Rumen and the Enzyme Properties

作　　者：张贝贝[1] 赵晓兵[1] 何丽丽 徐新慧[1] 刘新育[1] 陈红歌[1] 

机构地区：[1]河南农业大学生命科学学院/农业部农业微生物酶工程重点实验室,河南郑州450002 [2]鹤壁市淇滨区动物疫病预防控制中心,河南鹤壁458030

出　　处：《河南农业科学》2014年第4期128-132,共5页Journal of Henan Agricultural Sciences

基　　金：河南省重点科技攻关项目(132102110128)

摘　　要：为筛选能够产生稳定性较高木聚糖酶的菌株，采用以桦木木聚糖为唯一碳源的培养基，从牛瘤胃液中分离到1株产木聚糖酶的细菌，命名为BF。将BF菌株进行形态学分析、生理生化鉴定及16SrDNA鉴定，并研究其产木聚糖酶的最适反应温度、最适pH值、温度稳定性、pH值稳定性及金属离子对其酶活力的影响。结果显示，BF菌株属于类芽孢杆菌属（Paenibacillus）；BF菌株所产木聚糖酶反应最适温度为60℃，最适pH值为6．0，在pH值3．0～9．0稳定，在60℃保温180min后仍有65％的酶活力，具有较好的热稳定性；终浓度为5mmol／L的ca2＋、Fe2＋对酶活力有较强的促进作用，相对酶活力分别达130％和140％。表明，筛选到的菌株所产木聚糖酶具有较好的稳定性。In order to obtain a new xylanase with desirable properties,the bovine rumen fluid was collected to screen bacterial strains producing xylanase by using the selective medium with birch- wood xylan as the sole carbon source. A strain named BF which could produce xylanase was isola- ted and identified to the genus of Paenibacillus based on its morphological, physiological, and bio- chemical characteristics,as well as 16S rDNA phylogenetic analysis. The optimal pH of BF strain xylanase was 6. 0 and its optimal temperature was 60 ~C. The xylanase was stable in the wide range of pH value of 3.0-9.0 and could retained 65% relative activity after incubated for 180 min at 60 ℃, showing its good thermostability. Both Ca2＋ and Fez＋ with final concentration of 5 mmol/L had a strong promoting effect on xylanase activity,with relative activity of 130% and 140% ,respectively. The BF strain xylanase had a high industrial annlicatinn nntontinl

关 键 词：木聚糖酶 牛瘤胃 类芽孢杆菌 酶学性质 

分 类 号：S816.73[农业科学—饲料科学]