虎奶菇发酵液抑菌成分的理化性质及其分离鉴定  

Physical-chemical Properties,Isolation and Identification of Antimicrobial Substances from Pleurotus tuber-regium Fermenting Borth

作　　者：林陈强[1] 严军[2] 王衍霖[2] 陈济琛[1] 林新坚[1] 

机构地区：[1]福建省农业科学院土壤肥料研究所,福州350013 [2]福建农林大学生命科学学院,福州3500021

出　　处：《天然产物研究与开发》2014年第4期534-538,共5页Natural Product Research and Development

基　　金：福建省公益类科研院所项目(2011R1024-4);福建省科技厅重点项目(2013Y1003)

摘　　要：为探明虎奶菇发酵液中的抑菌成分，本文以金黄色葡萄球菌（Staphylococcus aureus）为指示菌，研究了温度、pH、紫外线、蛋白酶K对虎奶菇发酵液抑菌活性的影响；并采用硅胶柱层析及反相中压液相层析追踪分离抑菌活性物质，通过核磁共振并结合相关资料对分离到的单体化合物进行了结构鉴定。结果显示，发酵液抑菌成分对蛋白酶较为稳定，对紫外照射敏感，可在90℃以下、pH1—9的范围内较好的保持其抑菌活性；活性追踪分离得到1个抑菌物质PtrA，经鉴定为2-呋喃甲酸（2-furoicacid）。In order to look for the antimicrobial substances from Pleurotus tuber regium fermenting liquor, the effects of temperature, UV, pH changes and protease on the antimicrobial substances from Pleurotus tuber-regium fermenting broth were studied. Then the antimicrobial substances were isolated, purified by silica gel column chromatography and re-versed-phase medium-pressure liquid chromatography, and a kind of antimicrobial compound was identified by combi- ninglH NMR and 13^C NMR with related papers. The results show that the antimicrobial substances in fermenting liquor are stable when influenced by protease, sensitive to ultraviolet radiation and better activity below 90 ℃ and at pHI-9. Guided by antimierobial activity, a kind of antimicrobial compound PtrA was isolated and its structure was elucidated as 2-furoic acid.

关 键 词：发酵液 抑菌活性 理化性质 分离鉴定 

分 类 号：Q936[生物学—微生物学] Q586