黄芪对大鼠海马齿状回区氨基酸物质含量的影响  

Effect of Radix astragali on the concentrations of amino acids in hippocampal dentate gyrus in rats

作　　者：李光燮[1,2] 王师[1] 王丹[1] 李英顺[1] 金清华[1] 

机构地区：[1]延边大学医学院生理学与病理生理学教研部,吉林延吉133002 [2]延边大学临床学院

出　　处：《中国实验诊断学》2014年第4期526-529,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然基金资助课题(30960394;31160211)

摘　　要：目的观察黄芪水提液对大鼠海马齿状回(DG)区氨基酸物质的含量以及学习记忆能力的影响。方法Wistar系雄性大鼠24只,随机分为对照组、黄芪小剂量组及黄芪大剂量组。利用脑部微量透析法和高效液相色谱法,观察大鼠海马DG区细胞外液中的六种氨基酸的含量,包括天冬氨酸、谷氨酸、谷氨酰胺、甘氨酸、牛磺酸和丙氨酸,其后,用相应溶液灌胃3周,再次观察海马DG区六种氨基酸的含量,并利用穿梭箱观察大鼠的学习记忆能力。结果对照组海马DG区的六种氨基酸在灌胃前后无明显变化,而黄芪大、小剂量组海马DG区的牛磺酸含量灌胃后出现明显的增加(均为P<0.05);在5天的穿梭箱训练过程中,黄芪大、小剂量组的每天的训练所需时间逐渐缩短,与对照组相比有显著性差异(均为P<0.05)。结论黄芪增强大鼠的学习记忆能力,其作用机制可能与增加海马DG区牛磺酸含量有关。Objective To investigate the effects of Radix astragali on extracelluar levels of amino acids in the hipp-ocampal dentate gyrus （DG）region in rats.Methods Totally 24 Wistar rats were randomly divided into three groups, control group,Radix astragali low dose group and Radix astragali large dose group.The rats were intragastric adminis-trated with Radix astragali or saline 3w,and then learing and memory were detected by shuttle box.The extracellular concentrations of aspirate （Asp）,glutamate （Glu）,glutamine （Gln）,glycine （Gly）,taurine （Tau）and alanine （Ala）in the hippocampal DG were measured by microdialysis and high performance liquid chromatography （HPLC）techniques before and after of intragastric administration.Results Compared with control group,the extracellular concentrations of Tau in the hippocampal DG was significantly increased in the Radix astragali groups （P 〈0.05）.Moreover,the Radix astragali groups showed significantly shorter training time compared with control group （P 〈0.05）.Conclusion Radix astragali enhance the ability of learning and memory in rats,which maybe related to increase in Tau concentrations in the hippocampal DG region.

关 键 词：黄芪 海马齿状回 牛磺酸 学习记忆 大鼠 

分 类 号：R285.5[医药卫生—中药学]