Theanine improves the function of dendritic cells via the downregulation of cyclooxygenase-2 expression  

作　　者：Lei Mingsheng Zuo Jianhong Li Min Gu Qihua Hu Chengping 

机构地区：[1]Department of Respiratory and Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China [2]Department of Respiratory and Critical Care Medicine, Zhangiiajie City Hospital, Zhangjiajie, Hunan 427000, China [3]Medical School, University of South China, Hengyang, Hunan 421001, China

出　　处：《Chinese Medical Journal》2014年第8期1545-1549,共5页中华医学杂志（英文版）

基　　金：This work was supported by grants from the National Nature Science Foundation of China （No. 81272960）, Key Research Program from Science and Technology Department of Hunan Province, China （No. 2013WK2010）, Research Program from Education Department of Hunan Province, China （No. 12C0343）, Research Program from Health Department of Hunan Province, China （No. B2012-047） and the Funds for the Development of Chemical Industry of Forest Products in Htman Province Key Laboratory （No. JDLC201203）.

摘　　要：Background Tumor cells can reduce the number of dendritic cells （DCs） in the tumor environment and cause DC dysfunction through autocrine or paracdne pathways. We sought to measure cyclooxygenase-2 （COX-2） expression in bombesin-inhibited DCs treated with theanine in vitro and to explore the protection and activation effects of theanine on DCs. Methods Enzyme-linked immunosorbent assay （ELISA）, reverse transcription-polymerase chain reaction （RT-PCR）, and Western blotting were used to analyze the effects of theanine on COX-2 expression and interleukin （IL）-12/IL-10 secretion of bombesin-treated DCs. Results DCs acquired an impaired phenotype as a result of bombesin treatment. Theanine increased the expression of mature DC surface molecules. The number of cell apoptosis with the treatment of bombesin and theanine significantly decreased, accounting for 15.9%, compared with 26.1% of cell apoptosis with bombesin. COX-2 expression in bombesin- treated DCs was inhibited by theanine in a dose-dependent manner. Theanine promoted DC secretion of IL-12. IL-12 levels reached （137.4_＋4.9） pg/ml with theanine at 200 pmol/L. However, theanine inhibited the secretion of IL-10 in a dose-dependent manner. IL-10 levels were only （58.4_＋6.9） pg/ml with theanine at 200 IJmol/L. Conclusion Theanine inhibits the transcription and translation of COX-2 and regulates the balance of IL-10/IL-12 secretion in bombesin-inhibited DCs, leadincl to the recovery of a state of activation in DCs.Background Tumor cells can reduce the number of dendritic cells （DCs） in the tumor environment and cause DC dysfunction through autocrine or paracdne pathways. We sought to measure cyclooxygenase-2 （COX-2） expression in bombesin-inhibited DCs treated with theanine in vitro and to explore the protection and activation effects of theanine on DCs. Methods Enzyme-linked immunosorbent assay （ELISA）, reverse transcription-polymerase chain reaction （RT-PCR）, and Western blotting were used to analyze the effects of theanine on COX-2 expression and interleukin （IL）-12/IL-10 secretion of bombesin-treated DCs. Results DCs acquired an impaired phenotype as a result of bombesin treatment. Theanine increased the expression of mature DC surface molecules. The number of cell apoptosis with the treatment of bombesin and theanine significantly decreased, accounting for 15.9%, compared with 26.1% of cell apoptosis with bombesin. COX-2 expression in bombesin- treated DCs was inhibited by theanine in a dose-dependent manner. Theanine promoted DC secretion of IL-12. IL-12 levels reached （137.4_＋4.9） pg/ml with theanine at 200 pmol/L. However, theanine inhibited the secretion of IL-10 in a dose-dependent manner. IL-10 levels were only （58.4_＋6.9） pg/ml with theanine at 200 IJmol/L. Conclusion Theanine inhibits the transcription and translation of COX-2 and regulates the balance of IL-10/IL-12 secretion in bombesin-inhibited DCs, leadincl to the recovery of a state of activation in DCs.

关 键 词：THEANINE dendritic cells BOMBESIN CYCLOOXYGENASE-2 

分 类 号：R285[医药卫生—中药学]