Docosahexaenoic acid inhibits melanin synthesis in murine melanoma cells in vitro through increasing tyrosinase degradation  

作　　者：Marie Carmel BALCOS Su Yeon KIM Hyo-soon JEONG Hye young YUN Kwang Jin BAEK Nyoun Soo KWON Kyoung chan PARK Dong-seok KIM 

机构地区：[1]Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156–756, Korea [2]Department of Dermatology, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Kyoungki-do 463–707, Korea

出　　处：《Acta Pharmacologica Sinica》2014年第4期489-495,共7页中国药理学报（英文版）

基　　金：The studies were mainly supported by research grants from the National Natural Science Foundation of China （81073090 and 81274134）. We thank Prof Hou-kai LI （Shanghai University of Traditional Chinese Medicine） for his contribution to the English translation.

摘　　要：Aim： To investigate the effects of docosahexaenoic acid （DHA） on melanin synthesis and related regulatory mechanisms. Methods： B16F10 mouse melanoma cells were exposed to DHA for 3 d, and melanin content and tyrosinase activity were measured. Western blot analysis was used to analyze the protein levels in DHA-mediated signal transduction pathways.Results： DHA （1–25 μmol/L） did not affect the viability of B16F10 cells, but decreased α-MSH-induced melanin synthesis in a concentration-dependent manner. DHA concentration-dependently reduced tyrosinase activity in the cells, but did not affect mushroom tyrosinase activity in a cell-free system. Furthermore, DHA treatment significantly reduced tyrosinase level without affecting microphthalmia-associated transcription factor （MITF） in the cells. DHA did not activate ERK and Akt in the cells. Pretreatment with the proteasome inhibitor MG132 （80 nmol/L） abolished DHA-induced tyrosinase reduction. Conclusion： DHA inhibits melanogenesis in B16F10 cells in vitro through increasing tyrosinase degradation. The results suggest that DHA may be a potential agent for treatment of hyperpigmentary disorders of skin.Aim： To investigate the effects of docosahexaenoic acid （DHA） on melanin synthesis and related regulatory mechanisms. Methods： B16F10 mouse melanoma cells were exposed to DHA for 3 d, and melanin content and tyrosinase activity were measured. Western blot analysis was used to analyze the protein levels in DHA-mediated signal transduction pathways.Results： DHA （1–25 μmol/L） did not affect the viability of B16F10 cells, but decreased α-MSH-induced melanin synthesis in a concentration-dependent manner. DHA concentration-dependently reduced tyrosinase activity in the cells, but did not affect mushroom tyrosinase activity in a cell-free system. Furthermore, DHA treatment significantly reduced tyrosinase level without affecting microphthalmia-associated transcription factor （MITF） in the cells. DHA did not activate ERK and Akt in the cells. Pretreatment with the proteasome inhibitor MG132 （80 nmol/L） abolished DHA-induced tyrosinase reduction. Conclusion： DHA inhibits melanogenesis in B16F10 cells in vitro through increasing tyrosinase degradation. The results suggest that DHA may be a potential agent for treatment of hyperpigmentary disorders of skin.

关 键 词：docosahexaenoic acid Α-MSH MELANIN MELANOGENESIS TYROSINASE PROTEASOME MG132 skin hyperpigmentary disorders 

分 类 号：Q959.530.7[生物学—动物学] TS225.24[轻工技术与工程—粮食、油脂及植物蛋白工程]