高效液相色谱法检测淀粉及含淀粉食品中6种有机酸  

Determination of six organic acids in starchy food by high performance liquid chromatography

作　　者：王丹 吕冰 周爽 鲁杰 赵云峰 

机构地区：[1]国家食品安全风险评估中心,北京100021

出　　处：《中国食品添加剂》2014年第2期108-113,共6页China Food Additives

基　　金：质检总局公益性行业专项-食品中违禁成分精准判别及快速检测技术验证(2012104003-1)

摘　　要：研究了应用高效液相色谱法检测淀粉及淀粉类食品中顺丁烯二酸、反丁烯二酸、苹果酸、琥珀酸、酒石酸、乙酸等6种有机酸的测定方法.利用SAX色谱柱连接C18预柱的方法,实现了对上述有机酸的完全分离.研究了分析方法的线性、灵敏度和精密度,结果在所确定的实验条件下,6个有机酸的线性系数大于0.999,在淀粉及淀粉类食品中的加标回收率处于75％ ～95％之间,变异系数均小于5.0％.A High performance liquid chromatographic （HPLC） method of detecting six organic acids in starchy food was established.Maleic acid,fumaric acid,malic acid,succinic acid,tartaric acid and acetic acid were separated by a SAX column with a C18 pre-column.The linearity,detecting limit and accuracy of the method was studied.The linearity of the organic acids was over 0.999,the recovery of the method was between 75％ to 95％ and RSD was less than 5％.

关 键 词：淀粉食品 液相色谱法 有机酸 

分 类 号：TS207.3[轻工技术与工程—食品科学]