石柑子提取物体外抗氧化活性的研究  

The Study on Vitro Antioxidant Activity of Extracts of Pothos chinensis(Raf.) Merr

作　　者：黄琼[1] 施丽娟[1] 黄永春[1] 陈薇[1] 

机构地区：[1]广西科技大学生物与化学工程学院,柳州545006

出　　处：《食品工业》2014年第5期108-111,共4页The Food Industry

基　　金：广西工学院科学基金项目(院科自1166213);广西教育厅项目(201106LX394);2012年广西大学生创新创业计划项目(51)

摘　　要：为检测石柑子各种提取物的体外抗氧化活性。采用DPPH自由基清除法来评估石柑子各种提取物抗氧化活性,并用人工合成抗氧化剂VC和BHA为参照物。结果表明,石柑子不同提取物对DPPH自由基均具有一定的清除能力,并且随着质量浓度的增大,其清除率越高,清除效果越好,抗氧化能力越强。不同提取物其抗氧化性能强弱为:VC溶液>甲醇提取物>乙酸乙酯提取物>BHA溶液>乙醇提取物>石油醚提取物>水提提取物>挥发油。石柑子提取物中甲醇提取物清除DPPH自由基的效果最好,石柑子甲醇提取物、VC和BHA的IC50分别是1.83μg/mL,0.57μg/mL,3.12μg/mL。表明该植物提取物有较强的抗氧化活性,是一种天然抗氧化剂的重要来源。Examine the antioxidant activities of various extracts of whole plant ofPothos chinensis in vitro. The antioxidant activity was evaluated by the DPPH. assays, with the synthetic antioxidants VC and BHA as the reference substance. The results showed that the different extracts of the Pothos chinensis have certain clearance ability to the free radicals of the DPPH., and with the increase of concentration, the higher the clearance, the better of clearance and the stronger of the antioxidant capacity. The order of the antioxidant activities was that： VC solution〉methanol extracts〉ethyl acetate extracts〉ethanol extracts〉petroleum ether extract〉water extraction〉essential oil. The best clearance ability to the DPPH. was the methanol extracts ofPothos chinensis, the IC50 values of the methanol extracts ofPothos chinensis and VC and BHA were respectively 1.83 μg/mL, 0.57 μg/mL, 3.12 μg/mL. These in vitro assays indicate that this plant extracts have mood antioxidant activity and it is a significant source of natural antioxidant.

关 键 词：石柑子 抗氧化活性 DPPH· 

分 类 号：TQ460.1[化学工程—制药化工]