产聚β-羟基丁酸酯(PHB)菌种的筛选及16SrDNA鉴定  

Screening and 16SrDNA Identification about Poly-β-hydroxybutyrate Production Strain

作　　者：刘俊梅[1] 王璐[1] 李琢伟[2] 王丹[1] 王辉[1] 王庆[1] 杨盼盼[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]长春职业技术学院,吉林长春130033

出　　处：《粮油加工（电子版）》2014年第4期71-75,共5页Machinery for Cereals Oil and Food Processing Main Contents

基　　金：吉林省科学技术厅重大专项;项目编号:20116034

摘　　要：采用尼罗蓝荧光法结合苏丹黑染色法从污水中初筛获得产聚-β-羟基丁酸酯(PHB)的菌种,超声波破壁处理后采用氯仿法抽提PHB,最终通过气相色谱和紫外可见分光光度计确定了一株高产PHB菌株PK3,PHB产量为0.732g/L。经形态观察、16SrDNA序列分析,初步鉴定PK3为假单胞菌属(Pseudomonas koreensis)。Strains producing PHB were preliminary screening from sludge by Nile blue fluorescence staining combining with Sudan black. PHB was extracted with chloroform ＇after ultrasonic wall-breaking treatment. Finally, a high yield PHB strain named PK3 was determined by gas chromatography and uhraviolet spectrophotometer, yield of PHB was 0.732 g/L. Strain PK3 was identified as Pseudomonas koreensis by morphological observation and 16SrDNA sequence analysis.

关 键 词：聚-β-羟基丁酸酯 选育 检测 鉴定 

分 类 号：O631.3[理学—高分子化学]