酿酒酵母发酵条件与培养基组成的优化研究  

Optimization of Fermentation Conditions and Components of Culture Medium for Saccharomyces cerevisiae

作　　者：周小玲 周映华 高书锋 刘惠知 胡新旭 舒燕 王升平 曾艳 

机构地区：[1]湖南省微生物研究院,湖南长沙410009 [2]饲用微生态制剂湖南省工程试验室,湖南长沙410009

出　　处：《湖南农业科学（下半月）》2014年第4期69-72,共4页

基　　金：湖南省科技厅重点项目(2011NK2013);科技部成果转化资金项目(2013GB2D200338)

摘　　要：在单因素试验的基础上,进一步采用正交试验,对一株用于发酵饲料的酿酒酵母菌株进行了培养条件和培养基组成的优化试验。试验结果表明,该酿酒酵母菌株的最佳发酵条件为:接种量8%,培养基初始pH值6.5,转速250 r/min,发酵温度30℃,装液量20%,发酵时间30 h;最佳发酵培养基组成为:葡萄糖20 g/L,蛋白胨7.5 g/L,酵母粉7.5 g/L,磷酸二氢钾0.156 g/L,七水硫酸镁0.496 g/L。在上述发酵条件和发酵培养基下进行发酵,酿酒酵母活菌数可达8.4×108 CFU/mL。The fermentation conditions and the components of culture medium for Saccharomyces cerevisiae which used in fermenting feed were optimized by the single factor experiment and the orthogonal experiment. The results showed that the optimum fermentation conditions were 8% of inoculation proportion, initial pH of 6.5, speed of 250 r/min, fermentation temperature of 30℃, 50 mL of liquid medium in a 250 mL of erlenmeyer flask, 30 h of fermentation; the optimum fermentation medium for Saccharomyces cerevisiae was 20 g/L of glucose, 7.5 g/L of peptone, 7.5 g/L of yeast extract, 0.156 g/L of KH2PO4, and 0.496 g/L of MgSO4.7H2O. The broth viable count was 8.4×10^8 cfu/mL with the optimal fermentation conditions and the optimal medium conponents.

关 键 词：发酵饲料 酿酒酵母 发酵条件 培养基组成 筛选优化 

分 类 号：S663.1[农业科学—果树学]