海生红藻中藻蓝、别藻蓝蛋白的凝胶过滤分离  

Isolation of the phycocyanin and allophycocyanin from Marine Red Algae by gel filtration

作　　者：王玉[1] 赵明日[1] 龚雪琴[1] 孙力[1] 

机构地区：[1]烟台大学生命科学学院,山东烟台264000

出　　处：《食品工业科技》2014年第11期93-97,102,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(30571720;40976083)

摘　　要：选用Sephadex G-150(G-150)、Sephadex G-200(G-200)、Sephacryl S-200(S-200)和Sephacryl S-300(S-300)四种凝胶对富含藻红蛋白的多管藻藻胆蛋白提取液进行过滤,并对所得藻蓝、别藻蓝蛋白组分做进一步纯化,优化建立了有效分离去除藻红蛋白、纯化制备藻蓝、别藻蓝蛋白组分的技术方法,即依次使用G-150、S-300和S-200凝胶过滤,所得样品中藻蓝和别藻蓝蛋白相对含量显著提高。并且根据实验现象,对凝胶柱的选择性、柱效、内水体积以及样品的蛋白组成等主要因素与凝胶过滤实际效果的相互关系进行了论述。Gel filtration to the extracted phycobiliprotein solution from Polysiphonia urceolata rich in phycoerythrin was performed by four gels of Sephadex G- 150 （ G- 150）, Sephadex G-200 （ G-200）, Sephacryl S-200 （ S-200 ） and Sephacryl S-300（S-300） ,and the obtained fraction of phycocyanin and allophycocyanin was further purified by the gel filtration on G-150,S-300 and S-200.An optimized technical procedure of isolating phycocyanin and allophycocyanin from the extracted phycobiliproteins of Polysiphonia urceolata by the gel filtration in turn on G-150,S-300 and S-200 was established based on the experiments.By the procedure,the phycoerythrin was efficiently separated and the phycocyanin and allophycocyanin were purified to such a degree that the relative content of phycocyanin and allophycocyanin in the obtained fraction increased with respect to the extracted phycobiliproteins solution.The relationships of practical gel filtration effects with some main factors of gel column, such as selectivity, efficiency and the inside volume, and the constituents of protein samples were discussed on the basis of observed phenomena in the experiments.

关 键 词：凝胶过滤 多管藻 藻红蛋白 藻蓝蛋白 别藻蓝蛋白 

分 类 号：TS201.2[轻工技术与工程—食品科学]