HPLC-ESI-MS分析紫色小白菜中花色苷组成分析  

HPLC-ESI-MS analysis of the structure and content of anthocyanins in purple pakchoi

作　　者：徐学玲[1] 赵岫云[1] 王丹[1] 赵晓燕[1] 马越[1] 

机构地区：[1]北京市农林科学院蔬菜研究中心农业部华北地区园艺作物生物学与种质创制重点实验室农业部都市农业(北方)重点实验室,北京100097

出　　处：《食品工业科技》2014年第11期278-281,294,共5页Science and Technology of Food Industry

基　　金：北京市自然科学基金重点项目(6111001);现代农业产业技术体系建设专项资金(CARS-25);北京市农林科学院科技创新能力建设专项

摘　　要：采用HPLC-ESI-MS方法测定并分析"新紫冠"紫色小白菜中花色苷的组分及其含量。结果表明,"新紫冠"紫色小白菜中含有矢车菊及飞燕草两类花色苷,以矢车菊-3,5-双葡萄糖苷标准品测得总花色苷含量为51.36μg/g·fw,其中矢车菊类花色苷含50.71μg/g·fw,占总含量的98.73%,飞燕草类花色苷含0.65μg/g·fw,仅占1.27%。矢车菊类花色苷全部以酰基化的形式存在,共检测出10种矢车菊类花色苷及相应的9种同分异构体,其中矢车菊-3-阿魏酰-槐糖苷-5-丙二酰-葡萄糖苷是含量最多的花色苷成分,达16.36μg/g·fw,占总含量的31.85%。Contents and structure of anthocyanins in purple pakchoi were identified by high performance liquid chromatography-electrospray ionization tandem mass spectrometry （HPLC-ESI-MS）.Two kinds of anthocyanins were found in ＂New ZIGUAN＂ purple pakchoi.The mainly existence of anthocyanins were acylation form,there were 10 anthocyanins and 9 isomeric compounds.The total anthocyanin content of purple pakchoi was 51.36μg/g .fw,which was calculated as the reference of cyanin- 3,5- diglucoside standard.Among them, cyanidin content was 50.71μg/g.fw, accounting for 98.？3%.And delphinidin content was 0.651Jg/g.fw,accounting for 1.27%.Cyanidin-3- feruloyl-sophoroside-5-malonyl-glucosidase was the major anthocyanin,accounting for 31.85% （16.36μg/g·fw） of the total anthocyanins.

关 键 词：紫色小白菜 花色苷 HPLC-MS 

分 类 号：TS201.2[轻工技术与工程—食品科学]