青钱柳中α-葡萄糖苷酶抑制剂的研究  

Study on anα-glucosidase inhibitor separated from Cyclocaryapaliurus leaves

作　　者：沈兵[1,2] 赵巍[3] 欧文斌 周盛梅[5] 刘俊 孟凡国 

机构地区：[1]浙江大学生命科学学院,杭州310058 [2]嘉兴出入境检验检疫局,浙江嘉兴314001 [3]浙江工业大学生物与环境工程学院,杭州310014 [4]浙江清华长三角研究院浙江省应用酶学重点实验室,浙江嘉兴314006 [5]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《浙江大学学报（农业与生命科学版）》2014年第3期275-280,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省重大科技专项资助项目(2012C03007-4);浙江省应用酶学创新团队资助项目(2010R50019)

摘　　要：利用Sephadex LH-20柱层析和半制备液相色谱从青钱柳叶水提取物中分离得到一种易溶于水的白色粉末状化合物,得率约为0.03‰。紫外光谱和高分辨质谱表明该化合物具有多糖特性。该化合物对α-葡萄糖苷酶有很高的亲和性,反应时间小于0.5min。该化合物对α-葡萄糖苷酶有很强的抑制作用,半抑制质量浓度(IC50)为3.13μg/mL,抑制作用类型属于混合非竞争性抑制。Summary Cyclocarya paliurus （Batal.） Iljinskaja is a multiple-function tree species in China.Due to its hypotensive and hypoglycemic functions,C.paliurus leaves have been used to make beverage over a long period of time.Studies of C.paliurus （Batal.） Iljinskaja began in 1 970s in China.A variety of compounds,including flavonoids,terpenoids,alkaloids,saccharides,alcohol compounds,phenolic compounds and carboxylic acids,etc., have been separated and purified from C.paliurus leaves.Recently,hypoglycemic effects of crude extract from C. paliurus leaves in alloxan diabetic mouse have been reported.So far,only a few studies were performed to separate and analyze the active components from C.paliurus . The active components of C.paliurus leaves,which inhibit the activity of α-glucosidase,were separated and purified by using water extraction,alcohol precipitation,Sephadex LH-20 column chromatography and semi-preparative liquid chromatography.Finally,a white powder compound was obtained with yield of 0.03‰.The compound contained the characteristics of polysaccharide by analysis of its ultraviolet spectrum and high resolution mass spectrum,and showed a potent inhibitory effect against α-glucosidase activity with IC50 （the compound concentration of 50% inhibitory rate） of 3.13 μg/mL.The inhibition time of the compound against enzymatic＆amp;nbsp;activity was less than 0.5 min,indicating the high affinity between the inhibitor and the enzyme.The inhibition of enzymatic activity by the compound was hybrid non-competitive. In conclusion,the water extract of C.paliurus leaves and the separated powder compound with polysaccharide characteristics have potent inhibitory effects against α-glucosidase activity.

关 键 词：青钱柳 Α-葡萄糖苷酶 抑制剂 分离 酶抑制动力学 

分 类 号：Q946.3[生物学—植物学]