油菜蜂花粉保肝活性组分的分离及细胞学筛选  

The Separation and Cytological Screening of Hepatoprotective Components in Rape Bee Pollen

作　　者：彭定利[1,2] 孙丽萍[1,3] 庞杰[2] 徐响[1,3] 杜夏[1] 王馨[1] 葛苗苗[1,2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]福建农林大学食品科学学院,福州350002 [3]农业部授粉昆虫生物学重点实验室,北京100093

出　　处：《中国食品学报》2014年第3期100-105,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31172273);"十二五"国家科技支撑计划项目(2011BAD33B04);国家现代农业(蜂)产业技术体系建设专项(CARS-45KXJ18)

摘　　要：目的:研究油菜蜂花粉中具有保肝功效的活性组分。方法:以四氯化碳(CCl4)诱导人正常肝细胞(L-02)建立体外细胞损伤模型,采用MTT法(噻唑兰比色分析法)测定细胞存活率,并以其作为活性筛选指标,采用溶剂萃取、反相色谱层析作为分离手段,对油菜蜂花粉各组分进行细胞学筛选,结合薄层色谱层析法(TLC)进行检测。结果:体外细胞损伤模型的造模浓度以10 mmol/L CCl4为佳,细胞活性筛选结果显示正丁醇萃取物活性最高,100μg/mL和200μg/mL剂量组细胞存活率分别为60.65%和76.98%;正丁醇萃取物各洗脱组分中A2活性最高,100μg/mL和200μg/mL剂量组细胞存活率分别为76.66%和64.47%,为油菜蜂花粉中主要的保肝活性组分。A2的定性分析及前期研究结果表明其为黄酮醇苷类物质。To investigate the hepatoprotective components in rape bee pollen,a vitro model of L-02 hepatocytes injured by CCl4was established.Cell survival rate measured by MTT assay was considered as the screening criterion,solvent extraction and reversed phase column chromatography were used as the separation method.TLC was used as a detection and analysis method.The result showed that the appropriate concentration of CCl4that could induce hepatocytes injury was determined as 10 mmol/L.N-butanol extract group showed the highest hepatoprotective effect that the cell survival rate of 100 μg/mL group and 200 μg/mL group was 60.65% and 76.98% respectively.Component A2separated from N-butanol extract showed the highest hepatoprotective effect that the cell survival rate of 100 μg/mL group and 200 μg/ mL group was 76.66% and 64.47%.The result of qualitative analysis and earlier research of A2suggested that A2may be flavonoides.

关 键 词：油菜蜂花粉 四氯化碳 肝损伤 黄酮醇苷 

分 类 号：TS201.2[轻工技术与工程—食品科学]