我国部分地区市售巴氏杀菌乳中β-内酰胺酶含量调查  

Investigation of β-lactamases activity in pasteurized milk in parts of China

作　　者：周蕊[1] 丁颢[1] 周爽[1] 赵云峰[1] 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021

出　　处：《中国食品卫生杂志》2014年第3期209-212,共4页Chinese Journal of Food Hygiene

基　　金：国家自然科学基金(81001248);国家质检总局-公益性行业科研专项经费(2012104003)

摘　　要：目的 采用酶热生物传感器法对我国部分地区巴氏杀菌乳样品中β-内酰胺酶进行快速检测及本底含量调查。方法 在巴氏杀菌乳样品中预先加入一定量青霉素G,室温震荡反应3h,使样品中的β-内酰胺酶充分酶解青霉素G底物,直接通过酶热生物传感器测定青霉素G含量。根据反应前后样品中青霉素G含量的变化,间接计算巴氏杀菌乳样品中β-内酰胺酶的活性。2013年6～8月在13个省市采集巴氏杀菌乳样品106份,对其β-内酰胺酶含量进行本底调查。结果 该方法线性范围为4～20U/ml,检出限和定量限分别为2和4U/ml。全国106份样品的总检出率为3.8%,其中北京市的47份样品中β-内酰胺酶活性均﹤2U/ml；其他12个省市的59份样品中β-内酰胺酶活性均﹤4U/ml,其中4个样品在2～4U/ml之间。结论 我国13个省市106份巴氏杀菌乳样品的测定结果表明样品中β-内酰胺酶含量普遍较低,一定程度上表明我国巴氏杀菌乳中违法添加β-内酰胺酶的问题已得到较大改善。Objective To investigate the β-Lactamases activities in pasteurized milk in China based on an enzyme thermistor （ET） biosensor.MethodsCertain amount of penicillin G was incubated with the milk sample for 3h, then an aliquot of the mixture was directly injected into the ET system to give a signal corresponding to penicillin G remained. The amount of β-lactamase was calculated by the penicillin G consumed during incubation. 106pasteurized milk samples collected from 13provinces were tested. ResultsThe linear range of this method was 4-20U/ml with LOQ and LOD of 2and 4U/ml, respectively. The total detection rate was 3.8%. 47pasteurized milk products collected in Beijing showed negative results containing β-lactamase lower than 2U/ml （LOD）. 59products collected from 12other provinces contained β-lactamase lower than 4U/ml, among which 4products were within the range of 2to 4U/ml.ConclusionLow levels of β-lactamase were detected in 106milk samples collected from 13provinces in China, and it was suggested that the illegal use of β-lactamase was almost eliminated in China.

关 键 词：Β-内酰胺酶 酶热生物传感器 巴氏杀菌乳 青霉素G 

分 类 号：R155[医药卫生—营养与食品卫生学] TS252.7[医药卫生—公共卫生与预防医学]