结合换算因子的苯酚-硫酸法测定红茶菌培养液中水溶性多糖含量的研究  

Determination of Water Soluble Polysaccharides in Kombucha by Phenol-sulfuric Acid Method Combined with Conversion Coefficient

作　　者：范海涛[1,2] 张虎成[1] 曲伟[3] 王孟君[1,3] 刘欣欣[1] 王鹏飞[1] 

机构地区：[1]北京电子科技职业学院生物工程学院,北京100029 [2]军事医学科学院毒物药物研究所,北京100027 [3]北京联合大学,北京100012

出　　处：《天然产物研究与开发》2014年第5期713-715,744,共4页Natural Product Research and Development

基　　金：北京市属高等学校人才强教计划资助项目(PHR 201107151);北京市高等学校教学名师资助项目(PXM2014-014306-000075);北京市教委科技面上项目(SQKM201210858002)

摘　　要：红茶菌发酵属于细菌和酵母菌的联合发酵,发酵过程中能产生细菌和酵母菌多糖。本研究对红茶菌多糖进行精制,并采用经典苯酚-硫酸比色法测定红茶菌精制多糖和粗多糖的含量,计算红茶菌多糖相对于葡萄糖的换算因子,并进行方法学考察。红茶菌多糖-葡萄糖的换算因子f=5.93。结果表明该法简便、快速、准确,校正后的测定结果更接近真实值。Kombucha is a beverage traditionally produced by metabolic activity of yeasts and acetic acid bacteria, which produce bacteria polysaccharides or yeast polysaccharides. The traditional method for content determination of polysac- charides cannot reflect the true value of Kombucha soluble polysaccharides. In this study, the polysaccharides were ex- tracted and purified from culturing broth of Kombucha by conventional method including Sevag method of removing pro- tein, treatment with hydrogen peroxide and dialysis. The contents of purified and crude polysaccharides were determined by sulfuric-phenol method. The conversion coefficient of Kombucha refined polysaccharides to glucose was determined to be 5.93 and used in the content determination. The validated method for the determination of soluble polysaccharide in Kombucha was simple, rapid and accurate. The determination results after correcting with conversion coefficient was clos- er to the true value.

关 键 词：红茶菌 多糖 含量测定 换算因子 

分 类 号：R917[医药卫生—药物分析学] R931.6[医药卫生—药学]