联合靶向Ras和mTOR信号抑制HCC细胞生长  

Combined blocked of Ras and mTOR signaling inhibit HCC cell growth

作　　者：杨件新[1] 施超[1] 施海辉[1] 

机构地区：[1]江苏省启东市人民医院普外科,启东226200

出　　处：《南通大学学报（医学版）》2014年第2期89-91,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的 :探讨联合靶向Ras和mTOR信号在肝细胞癌(hepatocellular carcinoma,HCC)治疗中的价值。方法 :四甲基偶氮唑盐(methylthiazolyl tetrazolium,MTT)检测不同浓度Ras抑制剂(farnesylthiosalicylic acid,FTS)和(或)mTOR抑制剂雷帕霉素对肝癌细胞增殖的影响;流式细胞仪检测细胞凋亡;进一步研究两种药物联合对Balb/c小鼠肝癌移植瘤生长的影响。结果:FTS联合雷帕霉素更能抑制HepG2细胞增殖、抑制小鼠肝癌移植瘤的生长,诱导肝癌细胞凋亡。结论:Ras和mTOR信号在HCC治疗中具有联合靶向价值。Objective： To investigate the value of combined blockade Ras and mTOR signaling in the therapy of HCC. Methods： Specific Ras and/or mTOR inhibitors were used to inhibit Ras and mTOR relatively. Cell proliferation was assessed by using the MTT assay. Early apoptosis was detected by Annexin-V-FITC/propidium iodide double staining assay. The effects of the two drugs on HCC were also assessed in xenograft models. Results： Ras inhibitor FTS and mTOR inhibitor all inhibited HepG2 and Huh-7 cell growth, induced cell apoptosis. Conclusion： Con-target Ras and mTOR could markedly inhibited HCC cell growth in vitro and in vivo.

关 键 词：肝细胞癌 RAS MTOR 靶向治疗 

分 类 号：R735.7[医药卫生—肿瘤]