LRIG3反义寡核苷酸对宫颈癌Hela229细胞LRIG3、EGFR表达及增殖的影响  

Effect of LRIG3-targeted antisense oligonucleotides on proliferation and expressions of LRIG3 and EGFR in Hela229 cells

作　　者：郭历琛[1,2] 史惠蓉[1] 盛浴澜 张平安 张锐 

机构地区：[1]郑州大学第一附属医院妇产科,郑州450052 [2]上海市嘉定区中心医院妇产科,上海201800

出　　处：《郑州大学学报（医学版）》2014年第3期326-331,共6页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨LRIG3反义寡核苷酸(ASODN)对宫颈鳞癌Hela229细胞LRIG3、EGFR表达及增殖的影响。方法:利用阳离子脂质体介导法分别用150、200及250 mg/L的LRIG3 ASODN、正义寡核苷酸(SODN)及无关序列核苷酸(MSODN)转染Hela229细胞24、48及72 h,分别应用RT-PCR和Western blot法检测细胞LRIG3、EGFR mRNA及蛋白的表达情况,应用MTT法检测细胞增殖率。以常规培养的细胞作空白对照。结果:转染24、48、72 h后,ASODN组细胞增殖率均较空白对照组、SODN组、MSODN组明显增高(P<0.05),LRIG3 mRNA和蛋白表达降低(P<0.05),EGFR mRNA和蛋白表达增高(P<0.05);3个ASODN剂量组间比较,随着LRIG3 ASODN剂量的增加,细胞增殖率增高(P<0.05),LRIG3 mRNA和蛋白表达水平降低(P<0.05),EGFR mRNA和蛋白表达水平升高(P<0.05)。结论:LRIG3 ASODN可促进宫颈鳞癌Hela229细胞的增殖,该效应可能与抑制LRIG3表达、促进EGFR表达有关。Aim ： To observe the proliferation and expressions of LRIG3 and EGFR in Hela229 cells transfected with LRIG3-targeted antisense oligonucleotide （ASODN）. Methods： The ASODN, SODN and MSODN of LRIG3 at doses of 150, 200 and 250 mg/L were transfected into Hela229 cells by the cationic liposome respectively for 24, 48, and 72 h. RT-PCR and Western blot were used to detect the expressions of LRIG3 and EGFR, and MTT assay was used to examine the proliferation of Hela229 cells. The cells with normal incubation were the blank control. Results： Compared with the blank control group, SODN group and MSODN group, the proliferation rate of ASODN group was significantly higher（ P 〈 0.05 ） , the expressions of LRIG3 mRNA and protein decreased（ P 〈 0.05 ）, and the expressions of EGFR mRNA and protein in- creased with the increase of ASODN concentration（P 〈 0.05 ）. Conclusion： LRIG3 ASODN can promote the proliferation of Hela229 cells, which is related to inhibition of LRIG3 expression and promotion of EGFR expression.

关 键 词：LRIG3 反义寡核苷酸 EGFR 增殖 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]