桂枝提取物抑菌活性研究  

Study on antifungal activity of Ramulus cinnamomi

作　　者：周梦娇[1] 万春鹏[1] 陈金印[1] 

机构地区：[1]江西农业大学农学院江西省果蔬保鲜与无损检测重点实验室,江西南昌330045

出　　处：《食品工业科技》2014年第12期96-98,共3页Science and Technology of Food Industry

基　　金："十二五"科技支撑计划项目(2012BAD38B03-2);国家自然科学基金项目(31160343);江西省教育厅科技落地计划项目(111);江西省"赣博英才555工程(2012);农业科技成果转化资金项目(2011GB2C500017);江西省博士后科研择优资助项目(2013KY31);留学人员科技活动项目(人社厅函[2013]277号);中国博士后科学基金第54批面上项目(2013M541870)

摘　　要：以柑橘青、绿霉菌及猕猴桃灰霉菌等17种植物病原菌为指示菌，分别采用杯碟法、菌丝生长速率法对桂枝提取物的抑菌活性进行研究，结果表明桂枝提取物具有较广泛的抑菌谱，对这17种供试病原菌均表现出一定的抑制效果，其中对柑橘青、绿霉的抑菌图大小分别为（32．67±0．23）mm和（30．50±0．17）mm，最小抑菌浓度（MIC）分别为3．125mg／mL和6．25mg／mL，最小杀菌浓度（MFC）分别为6．25mg／mL和12．5mg／mL，而在其他15种植物病原菌中，桂枝提取物对车前草穗枯菌的活性最强，半抑制浓度（EC_50）为0．4386mg／mL，对其他病原菌的EC。为1～22mg／mL。Seventeen kinds of phytopathogens such as Penicillium italicum, Penicillium digitatum and Botrytis cinerea were used as indicators to explore the antifungal activities of Ramulus cinnamomi by cyiind-plate and mycelial growth rates methods,respectively. The results showed that Ramulus cinnamomi had a broader spectrum antifungal activity against the 17 sorts of tested phytopathogens ,the inhibition zones against P.italicum and P.digitaturn were （32.67±0.23）mm and （30.50±0.17）mm,respectively. While the minimum inhibitory concentrations（MIC） gainst P.italicum and P.digitatum were 3.125mg/mL and 6.25mg/mL,respectively, and the minimum fungicidal （MFC） concentrations were 6.25mg/mL and 12.5mg/mL, respectively. Meanwhile, the toxicity of indoor test of Ramulus cinnamomi against the other 15 phytopathogens showed that Ramulus cinnamomi extracts had the best antifungal activity on Diaporthe angeficae with the ECso value of 0.4386mg/mL. The antifungal activities against other tested pathogens with ECso values ranged from 1 to 22mg/mL.

关 键 词：桂枝 植物病原菌 抑菌活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]