黄灯笼椒中高纯度辣椒碱类化合物的制备研究  

Preparation of high purity capsaicin from Capsicum chinense

作　　者：段宙位[1] 窦志浩[1] 张容鹄[1] 谢辉[1] 何艾[1] 

机构地区：[1]海南省农业科学院农产品加工设计研究所,海南海口571100

出　　处：《食品工业科技》2014年第12期277-281,286,共6页Science and Technology of Food Industry

基　　金：海南省科学事业费项目(11-21401-0006)

摘　　要：以辣椒油树脂为原料,研究树脂分离与结晶条件对辣椒碱类化合物纯度的影响。结果表明:NKA-Ⅱ大孔树脂对辣椒油树脂的分离效果最好,最佳吸附条件为:样品浓度4.0mg/mL,pH7,上样流速2.5mL/min;最佳洗脱条件为:洗脱剂90%乙醇,pH4,洗脱流速2.0mL/min。NKA-Ⅱ树脂分离辣椒油树脂后,经石油醚、乙醚两次萃取,得到辣椒碱类化合物粗品,用石油醚溶解,配成浓度为20mg/mL的溶液,在-5℃条件下重结晶3.0h,得到纯度为92.46%的辣椒碱类化合物。Capsicum oleoresin was used as the raw material to explore the effect of resin separation and crystallization on the purity of capsaicinoids. The results showed that NKA- Ⅱ macroporous resin was feasible for the purification. During the resin adsorption process,the optimum adsorption conditions were as follows：4.0mg/mL of the sample concentration,2.5mg/mL of the sample flow rate with pH7. And the optimal desorption conditions were 90% of alcohol,2.0mL/min of the eluting rate with pH4. After capsicum oleoresin was separated by NKA- Ⅱ resin and extracted by petroleum ether and ethyl ether,then it was recrystallized by petroleum ether for 3.0h with 20mg/mL of concentration and -5℃ of temperature,the purity of capsaicin was up to 92.46%.

关 键 词：黄灯笼椒 辣椒油树脂 辣椒碱类化合物 树脂 结晶 

分 类 号：TS202.3[轻工技术与工程—食品科学]