薏苡仁醇溶蛋白水解工艺优化及其ACE抑制活性的研究  

Optimization on Hydrolysis Conditions of Coix Prolamin and Angiotensin-converting Enzyme Inhibitory Activity of Hydrolysates

作　　者：袁建娜[1] 陈秀梅[1] 崔帅[1] 梁英喜 王忠雷[1] 张小华[1] 王灵芝[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《世界科学技术-中医药现代化》2014年第4期806-810,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然基金委青年基金项目(81102750):薏苡仁生物活性肽的获得及其生物学功能研究;负责人:王灵芝;北京中医药大学中药信息工程科研创新团队(CXTD-11);负责人:乔延江

摘　　要：目的:对薏苡仁中含量最丰富的醇溶蛋白水解条件进行优化,并对其水解产物进行血管紧张素转化酶(ACE)体外活性评价。方法:利用胃蛋白酶水解薏苡仁醇溶蛋白,邻苯二甲醛法测定水解度,通过正交实验优化醇溶蛋白酶解条件。酶解液超滤(截留分子量3 kD)冻干得到小分子量多肽,采用高效液相色谱法对其ACE抑制活性进行测定。结果:薏苡仁醇溶蛋白酶解的最佳条件为:底物浓度1%,酶与底物浓度比1∶5,水解时间48 h;酶解物在0.1 mg·mL-1浓度下体外ACE抑制率为83.40%±0.93%。结论:醇溶蛋白水解产物表现出较强的体外ACE抑制作用,为薏苡仁降压作用的进一步研究及功能食品的开发提供了理论支持。This study was aimed to optimize the hydrolysis conditions of Coix prolamin, which occupied the highest content in total protein of it, and then evaluate the angiotensin-converting enzyme （ACE） inhibitory activity of the corresponding hydrolysates. Pepsin was used to hydrolyze prolamin and OPA method was applied to determine the degree of hydrolysis. The optimum conditions of hydrolyzing prolamin were obtained by orthogonal design subse-quently. The ACE inhibitory activity of small molecular weight （less than 3 kD） peptides gained by ultrafiltration of the hydrolysates was assayed by RP-HPLC method. The result showed that the optimized hydrolysis conditions were substrate concentration of 1%, enzyme-to-substrate ratio of 1：5 and hydrolysis duration for 48 h. The hydrolysates exhibited an ACE inhibitory ratio of （83.40 ±0.93）% at the concentration of 0.1 mg·mL-1. It was concluded that prolamin hydrolysates displayed a high ACE inhibitory activity in vitro, which provided guidance for further research of antihypertensive component and development of functional food from Coix.

关 键 词：薏苡仁 醇溶蛋白 水解 ACE抑制活性 

分 类 号：R284[医药卫生—中药学]