高效液相色谱法测定面粉中偶氮甲酰胺的含量  

Determination of azodicarbonamide in flour by high performance liquid chromatography

作　　者：向露[1] 易廷辉 王雅[1] 王俊苏[3] 彭涛[4] 王国民[3] 母昭德[1] 

机构地区：[1]重庆医科大学药学院药物分析教研室,重庆400016 [2]重庆市农业委员会农业环境监测站,重庆401121 [3]重庆出入境检验检疫局、进出口食品安全工程技术研究中心,重庆400020 [4]中国检验检疫科学研究院农产品安全中心,北京100123

出　　处：《重庆医科大学学报》2014年第4期533-536,共4页Journal of Chongqing Medical University

基　　金：国家“十二·五”科技支撑计划资助项目(编号:2012BAD 33B02、2012BAK08B01);国家质检公益性行业科研专项基金资助项目(编号:201210016、201210086)

摘　　要：目的：建立面粉中偶氮甲酰胺（azodicarbonamide，ADc）的高效液相色谱检测方法。方法：样品经丙酮提取，正己烷脱脂，进入高效液相色谱后采用Eclipse XDB—CN柱（250mm×4．6mm，5μm）进行分离，以乙腈-20mm01／L乙酸铵为流动相进行梯度洗脱，二极管阵列检测器检测波长为245nm。结果：ADC在1．0—90．0mg/kg范围内线性关系良好，相关系数（r）大于0．99，定量限为5mg／kg（S／N≥10），在5．0、10．0、45．0mg／kg3个添加水平下回收率在68．6％～116．9％之间，相对标准偏差均小于7．0％。结论：建立的面粉中ADC液相色谱方法简单快捷，准确可靠，适用于面粉中ADC的测定。Objective：To develop an analytical method based on high performance liquid chromatography （HPLC） for the determination of azodicarbonamide （ADC） in flour. Methods ： Samples were extracted with acetone,degreased with n -hexane,then separated with a agilent Eclipse XDB-CN column（250 mm× 4.6 mm,5 μm） using acetonitrile-ammonium acetate （20 mmol/L） as mobile phase with a gradient elution program and were detected with diode array detector with the wavelength of 245 nm. Results：ADC had a good linear relationship within the range of 1.0-90.0 mg/kg;the correlation coefficient was higher than 0.99 and the limit of quantification（S/N ≥ 10） was 5.0 mg/kg. Average recoveries of ADC at spiking levels of 5.0,10.0,45.0 mg/kg were ranged from 68.6% to 116.9% and relative standard deviations were less than 7.0%. Conclusions：The method developed is simple,rapid, accurate and reliable;it can be used for the determination of ADC in flour.

关 键 词：偶氮甲酰胺 面粉 高效液相色谱法 测定 

分 类 号：O657.7[理学—分析化学]