解淀粉芽孢杆菌胞外多糖的理化性质及免疫调节作用  

Physicochemical properties and immunomodulatory effects of exopolysaccharide produced by Bacillus amyloliquefaciens

作　　者：范熠[1] 刘丽莎[1] 韩玉竹[1] 李平兰[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国农业大学学报》2014年第1期131-136,共6页Journal of China Agricultural University

基　　金：公益性行业(农业)科研专项经费项目(201303014-4);国家自然科学基金资助项目(31271827);国家大学生创新性实验计划项目(201210019088)

摘　　要：为了解EPS的粒径、热稳定性、乳化特性及流变学特性,以实验室选育的一株胞外多糖(EPS)高产菌株(Bacillus amyloliquefaciens LPL061)为材料,并考察EPS的体外免疫调节功能.结果表明:芽孢杆菌EPS粒径大小为445.6 nm;熔融温度为265.18℃;对食用油乳化效果较好,乳化指数达50％以上;EPS溶液为典型的假塑性流体,在20～80℃范围内黏度变化较小,具有较好耐温性;EPS能促进小鼠脾淋巴细胞增殖及细胞因子产生,具有一定的免疫活性.In this study,the particle size,thermal stability,rheological properties,emulsifying properties and immunomodulatory effects of exopolysaccharide of a high-yield strain Bacillus amyloliquefaciens LPL061 were studied and compared with those of xanthan gum.The results showed that the molecular size of EPS was 445.6 nm,the melting temperature is 265.18 ℃.LPL061 produced more stable emulsions with edible oils than xanthan gum.EPS also showed pseudoplastic non-Newtonian fluid behaviour in an aqueous solution,with thermal stability between 20 to 80 ℃.The EPS from LPL061 showed potential of immunomodulatory effects by promoted the lymphocyte proliferation of mouse.

关 键 词：解淀粉芽孢杆菌 胞外多糖 理化特性 免疫调节 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ925.1[轻工技术与工程—食品科学与工程]