血管生成抑制因子SCAIF80及其抗肿瘤效应  

SCAIF80, a Novel Inhibitor of Angiogenesis, and Its Effect on Tumor Growth

作　　者：沈先荣[1] 吉冬梅[1] 胡幼卿[1] 贾福星[2] 王玲[2] 储智勇[2] 任大明[1] 邓新贤 黄建生 雷呈祥 刘卫平 胡忻然 

机构地区：[1]复旦大学遗传学研究所 [2]海军医学研究所,上海200433

出　　处：《生物化学与生物物理学报》2001年第1期99-104,共6页

基　　金：总后九五科研基金资助项目;上海市现代生物技术与医药发展基金资助项目&&

摘　　要：从鲨鱼软骨中提取、纯化出一种新的血管生成抑制因子———鲨鱼软骨血管生成抑制因子 80 (sharkcarti lage derivedangiogenesisinhibitoryfactor 80 ,SCAIF80 ) ,SDS PAGE银染分析显示一条带 ,分子量为 80kD。利用血管内皮细胞增殖、迁移抑制实验 ,证明SCAIF80能显著抑制血管内皮细胞的增殖与迁移 ,均有剂量依赖性。采用鸡胚绒毛尿囊膜分析 ,证明SCAIF80能显著抑制新生血管生成。动物抑瘤实验结果表明SCAIF80显著抑制小鼠肿瘤生长 ,对Lewis肺癌的抑瘤率达 93 .83%。上述结果提示鲨鱼软骨含有一种新的蛋白质 ,可抑制新生血管生成 。A novel inhibitor of angiogenesis named SCAIF80 (shark cartilage derived angiogenesis inhibitory factor) from shark cartilage has been isolated and characterized. SDS PAGE analysis followed by silver staining revealed a single band with molecular weight ( M r) of 80 kD. To determine whether this protein was capable of inhibiting angiogensis, it was assayed in endothelial cell (EC) proliferation and migration assay. The results showed that SCAIF80 significantly suppressed EC proliferation and migration in a dose dependent manner. In the chick chorioallantoic membrane (CAM) assay, SCAIF80 also showed a potent inhibitory activity on angiogenesis in vivo . In animal tests, the growth of tumor was potently suppressed by SCAIF80 therapy. Lewis lung carcinoma was inhibited by 93.83 % at a dose of 5 mg/(kg·d). These findings suggest that shark cartilage may produce a novel protein with anti angiogenic and anti tumor activity.

关 键 词：SCAIF80 血管生成 鲨鱼软骨 鸡胚绒毛尿囊膜 肿瘤治疗 

分 类 号：R730.2[医药卫生—肿瘤]