烷烃对P450酶的诱导及二元酸发酵工艺改进  

Study on Fermentation of Long-chain Dicarboxylic Acid Based on Enzymology

作　　者：肖云智[1] 焦鹏[1] 华玉涛[1] 曹竹安[1] 

机构地区：[1]清华大学化工系生物化工研究所,北京100084

出　　处：《生物工程学报》2001年第2期218-220,共3页Chinese Journal of Biotechnology

基　　金：国家"九五"科技攻关项目! (96 C0 3 0 4 0 2 ) ;中国自然科学基金!项目 (30 0 0 0 0 0 3)和 (2 9976 0 2 2 ) ;中国自然科学重点

摘　　要：In this paper,we studied the effects of the adding time of alkane on the expression of P450 and production of dicarboxylic acid.A novel fermentation process,in which no or a little alkane was added to make the cells growing more quickly during the growth stage,followed by the addition of alkane to induce cytochromes P450 for 6～8 hours,was established.The results showed that the new process was much better than the old process on inductivities of cytochromes P450 and production of dicarboxylic acid.The new process improved nearly 14.56% expression of P450 and 14.15% production of dicarboxylic acid.In this paper,we studied the effects of the adding time of alkane on the expression of P450 and production of dicarboxylic acid.A novel fermentation process,in which no or a little alkane was added to make the cells growing more quickly during the growth stage,followed by the addition of alkane to induce cytochromes P450 for 6～8 hours,was established.The results showed that the new process was much better than the old process on inductivities of cytochromes P450 and production of dicarboxylic acid.The new process improved nearly 14.56% expression of P450 and 14.15% production of dicarboxylic acid.

关 键 词：长链二元酸 热带假丝酵母 细胞色素P450 烷烃 发酵工艺 

分 类 号：TQ921[轻工技术与工程—发酵工程]