Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro  

作　　者：Xin Chen Yongjie Zhu Lulu Han Hongting Lu Xiwei Hao Qian Dong 

机构地区：[1]Department of Pediatric Surgery,the Affiliated Hospital of Qingdao University [2]Operating Room,the Affiliated Hospital of Qingdao University

出　　处：《Neural Regeneration Research》2014年第10期1063-1067,共5页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81272986;the Natural Science Foundation of Shandong Province,No.ZR2011HZ002

摘　　要：This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.

关 键 词：nerve regeneration chemokine receptor 4 small interfering RNA NEUROBLASTOMA inva-sion Transwell chamber LIPOSOME NSFC grant neural regeneration 

分 类 号：R739.4[医药卫生—肿瘤]