地衣芽孢杆菌β-甘露聚糖酶的制备  

Preparation of β-Mannanase from Bacillus licheniformis

作　　者：张峻[1] 何志敏 胡鲲[1] 冯耀宇[1] 张志钢[1] 

机构地区：[1]天津大学化学工程研究所,天津300072 [2]天津渤海化工集团公司,天津300040

出　　处：《食品与发酵工业》2001年第2期5-7,共3页Food and Fermentation Industries

基　　金：天津市重大科技攻关资助项目! (编号 :97310 30 11)

摘　　要：提出了一种地衣芽孢杆菌β 甘露聚糖酶制备的工艺。结果表明 :在设定的操作条件下 ,6 6L自控罐发酵酶活可达 2 6 0u/mL。发酵液用AlCl3与壳聚糖絮凝预处理后 ,絮凝体喷雾干燥可制成 90 0u/g的酶粉 ,适合饲料和造纸工业使用 ;絮凝上清液先以平均截留分子质量5万的中空纤维聚砜膜分离 ,透过液再用平均截留分子质量 1万的中空纤维聚砜膜浓缩后 ,喷雾干燥可制成 110 0 0u/g的酶粉 ,适合食品和医药工业使用。以上工艺酶活总收率 88% 。A process has been developed for the preparation of β mannanase from Bacillus licheniformis  The enzyme activity was 260?u/mL when incubated in a 6 6L fermentor under the designed conditions After pretreatment of culture broth using flocculants of AlCl 3 and chitosan, the floc was spray dried (900?u/g) for use in feed or pulp industries, while the supernatant was fractionated by polysulfone membrane and then concentrated by polysulfone membrane The final product was spray dried (11?000?u/g) for use in food or pharmaceutical industries The proposed process resulted in the total yield of β mannanase activity of 88% and is easily scale up

关 键 词：地衣芽孢杆菌 Β-甘露聚糖酶 制备 发酵 酶活 混合絮凝 超滤 

分 类 号：TQ92[轻工技术与工程—发酵工程]