响应面法优化多棘海盘车消化腺、生殖腺酶解工艺  

Application of Response Surface Methodology on Enzymatic Hydrolysis of Digestive Gland and Gonad of Asterias amurensis

作　　者：曹荣[1] 张媛 刘淇[1] 李幸[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,碳汇渔业实验室,山东青岛266071 [2]獐子岛集团股份有限公司,辽宁大连116001

出　　处：《食品研究与开发》2014年第9期76-80,共5页Food Research and Development

基　　金：中央级公益性科研院所专项资金项目(20603022011005)

摘　　要：多棘海盘车在我国黄海、渤海海域分布广泛,严重危害贝类养殖。为丰富其高值化加工利用的基础理论,本实验以多棘海盘车消化腺、生殖腺为研究对象,以水解度为主要指标,结合酶解液风味分析,对水解用蛋白酶的种类进行了筛选。在此基础上,进行了酶解工艺的单因素分析,并采用响应面中心组合设计建立了二次回归模型。结果表明,中性蛋白酶适宜作为水解用酶,底物质量浓度、酶用量和水解时间对水解度影响较大,所建立的二次回归模型经方差分析和实验验证,证实能够较好地反映多棘海盘车消化腺、生殖腺水解度的变化规律,最佳的水解工艺条件为:底物质量浓度11.0%、酶用量1 768 U/g(以原料计)、水解时间140 min,在此条件下水解度可达45.67%。Asterias amurensis are widely distributed in China, and endanger shellfish aquaculture seriously. To enrich the basic theory of high-valued processing of starfish, enzymolysisexperiment was designed and carried out with digestive gland and gonad as the research object, and degree of hydrolysis as the main index. Protease screening was done according to degree of hydrolysis and flavor analysis. Then, effect of single factorssuch as substrate concentration, dosage of enzyme, hydrolyzation time, pH and temperature on the degree of hydrolysis was analyzed. Central composite design was carried out to optimize the hydrolysis process and quadratic regression model was established. Results showed that neutral protease is suitable as the hydrolysis enzyme. Substrate concentration, enzyme dosage, and hydrolyzation time have significant influence on the degree of hydrolysis.The quadratic regression model could reflect the hydrolyzation process, which was confirmed by variance analysis and the experimental verification. Based on response surface analysis, the optimum conditions were obtained as follows：substrate concentration of 11.0%, enzyme dosage 1768 U/g material, and hydrolyzation time of 140 min, under which the degree of hydrolysis could be 45.67%.

关 键 词：多棘海盘车 酶解工艺 响应面优化 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]