植物乳杆菌M1-UVs29体外降解胆固醇的作用  

Degradation of cholesterol with Lactobacillus plantarum M1-UVs29 in vitro

作　　者：郭峰[1] 樊磊[1] 廖宇欣[1] 钱丽丽[1] 于长青[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2014年第6期803-806,共4页Chinese Journal of Biologicals

基　　金：国家农业科技成果转化资金(2012GB2B200125);黑龙江省自然基金重点项目(ZD101207)

摘　　要：目的探讨植物乳杆菌M1-UVs29在不同条件下降解胆固醇的能力。方法将M1-UVs29干菌粉活化后,分别接种至MRS液体培养基、MRS-胆盐培养基、MRS-胆固醇培养基和MRS-胆盐-胆固醇培养基中培养,绘制M1-UVs29在不同培养基中的生长曲线;以胆固醇降解率为指标,考察M1-UVs29在不同条件下(胆固醇、胆盐添加量及菌种数量)降解胆固醇的能力。结果胆盐具有抑制菌体生长的作用,胆固醇具有缓解胆盐对菌体生长抑制的作用。随着培养基中胆固醇含量的逐渐增加,胆固醇降解率也随之升高,趋势相对稳定,培养基中胆盐含量为0.2%,胆固醇含量为400μg/ml时,胆固醇降解率可达34.76%;随着培养基中胆盐含量的逐渐增加,胆固醇降解率也增加,但不呈规律性变化,培养基中胆固醇含量为100μg/ml,胆盐含量为0.4%时,胆固醇降解率可达32.85%;培养基中胆盐含量大于0.5%,胆固醇降解率极剧下降;培养基中胆固醇含量为100μg/ml,胆盐含量为0.2%,菌种数量为2×108cfu/ml时,胆固醇降解率达最高,为37.30%。结论植物乳杆菌M1-UVs29具有稳定的降解胆固醇的能力,本实验为其进一步应用提供了参考。Objective To investigate the ability of Lactobacillus plantarum M1-UVs29 in degradation of cholesterol under various conditions. Methods The dry powder of M1-UVs29 was activated and inoculated to liquid MRS, MRS-bile salt, MRS-cholesterol and MRS-bile salt-cholesterol media respectively, of which the growth curves were plotted. The abilities of M1-UVs29 in degradation of cholesterol under various conditions （amounts of cholesterol and bile salt added and bacte- rial seeds inoculated） were evaluated using the degradation rate of cholesterol as an index. Results Bile salt showed in- hibitory effect on the growth of M1-UVs29, while cholesterol remitted the inhibitory effect. The degradation rate of choles- terol increased stably with the cholesterol content in medium. When the bile salt content was 0. 2%, and the cholesterol content was 400 μg/ml, the cholesterol degradation rate reached 34. 76%. However, the cholesterol degradation rate in- creased irregularly with the increasing bile salt content. When the cholesterol content was 100 μg/ml, and the bile salt content was 0. 4%, the cholesterol degradation rate reached 32. 85%. when the bile salt content was more than 0. 5%, the cholesterol degradation rate decreased remarkably. When the cholesterol content in medium was 100 μg/ml, bile salt content was 0. 2%, and the amount of bacterial seeds was 2 × 10^8 cfu/ml, the cholesterol degradation rate reached the maximum of 37. 30%. Conclusion L. plantarum M1-UVs29 showed stable ability in degradation of cholesterol, which provided a reference for its further application.

关 键 词：植物乳杆菌 M1-UVs29 胆固醇 降解 

分 类 号：R378.992[医药卫生—病原生物学] Q591.5[医药卫生—基础医学]