固定化里氏木霉制备纤维素酶的研究  

CELLULASE PRODUCTION BY IMMOBILIZED TRICHODERMA REESEI CELLS

作　　者：夏黎明[1] 余世袁[1] 程芝[1] 

机构地区：[1]南京林业大学化学工程系

出　　处：《南京林业大学学报（自然科学版）》1993年第2期1-6,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金资助项目 No.29170269

摘　　要：采用多孔塑料吸附固定里氏木霉(Trichoderma reesei Rut C30)菌丝细胞,以硫酸盐纸浆为底物,重复分批发酵生产纤维素酶,滤纸酶活力可达2.4IU·mL^(-1),纤维二糖酶活力为0.2IU·mL^(-1),分别是游离细胞发酵的1.6倍和2.2倍。固定化菌丝细胞性状稳定,连续使用40 d以上,未见酶活力下降。酶解试验表明,固定化细胞生产的酶液对木质纤维原料具有很高的降解效率,当每克底物的酶用量在滤纸酶活力20 IU以上时,酶解得率可达90％以上。Mycelia of Trichoderma reesei Rut C30 were adsorbed and immobilized effectively on pieces of porous plastics. In repeated batch fermentations using sulphate pulp as the substrate of cellulase production, the filter paper and cellobiase activity produced by the immobilized cells were 2. 4 IU·mL-1 and 0. 2 IU·mL-1 respectively, which were 1. 6 times and 2. 2 times as high as that of the free cell fermentation separately. The immobilized cells were stable after having been continuously used for more than 40 days. The results of enzymatic hydrolysis have shown that the hydrolytic ability of the enzyme which was produced by the immobilized cells was very effective to lignocellulosic materials, when the enzyme dosage per gram substrate was above 20 IU, the efficiency of enzymatic hydrolysis was over 90 percent.

关 键 词：固定化细胞 里氏木霉 纤维素酶 多孔塑料 

分 类 号：TQ925.9[轻工技术与工程—发酵工程] Q814.2[生物学—生物工程]