肝癌中c-fms癌基因突变及其临床意义  

Mutation and clinical significance of c-fms oncogene in hepatocellular carcinoma

作　　者：崔俊[1] 杨冬华[1] 覃汉荣[1] 

机构地区：[1]中国人民解放军第一军医大学珠江医院消化科,广东省广州市510282

出　　处：《世界华人消化杂志》2001年第4期392-395,共4页World Chinese Journal of Digestology

基　　金：广东省自然科学基金;No.990422~~

摘　　要：目的探讨c-fms癌基因与肝细胞癌变的关系及意义,为进一步阐明肝癌的发生机制提供依据。方法设计两对引物分别分析c-fms癌基因编码产物CSF-IR(集落刺激因子1受体)第301,969位密码子。引物对1:上游(F):5-GAATTCGCCA GATGCTTGTG TGTTCTGC-3,下游(R):5-GTCGACGTCA CCTCCTGGAT GAGGT-3;引物对2:上游(F):5-GAATTCGACT ATACCAATCT GCCGAC-3;下游(R):5-GTCGACATG GTACTCCCTG TCGTCA-3。行PCR-SSCP,紫外透射下切割电泳行为异常的单链条带,以此异常单链条带为模板行PCR扩增产物克隆入T载体测序,对肝癌组织行Edmondson分级。结果 PCR扩增产物长度与预期一致,特异性高。SSCP检测发现3例肝癌组织第301位密码子DNA单链出现泳动变位,第969位密码子片段未出现异常泳动单链。测序示CSF-IR第301位密码子发生亮氨酸Leu(TTG)→丝氨酸Ser(TCG)置换,发生该位点突变的肝癌组织学分类为Edmondson Ⅲ级1例、Ⅳ级2例、具有恶性程度高、发病年龄轻的特点。结论 c-fms癌基因第301位密码子突变是肝癌发生发展的一种分子机制。AIM To study the clinical significance and relationship between c-fms oncogene and hepatocellular carcinogenesis, to further clarify the pathogenesis of hepatocellular carcinoma (HCC). METHODS Two pairs of primers were designed to analyse the 301st and 969th codon of CSF-IR (c-fms encoded protein). Primer pair Ⅰ: Forward (F): 5-GAATTCGCCA GATGCTTGTG TGTTCTGC-3, Reward(R):5-GTCGACGTCA CCTCCTGGAT GAGGT-3; Primer pair 2. Forward(F): 5-GAATTCGACT ATACCAATCT GCCGAG-3;Reward(R):5-GTCGACAGTG GTACTCCCTG TCGTCA-3. PCR-SSCP was performed, abnormal single strand band was cut off under ultraviolet, PCR was performed using the abnormal single band as template, PCR product was cloned into T vector to be sequenced. Histological classification was based on Edmondson standards Ⅰ Ⅱscale. RESULTS The lengths of PCR products were consistent with the prediction with high specificity. SSCP showed two abnormal single strands in 3 HCC tissues of 301st codon PCR products. No abnormal single strands were observed in the 969th codon PCR products. DNA sequencing showed transition of Leu (TTG)→Ser (TCG) at 301st codon of CSF-1R. Histological classification of the tumors were Ⅰ in Edmondson Ⅲ and 2 in Edmondson Ⅱ. The tumors had the characters of high malignancy and young age. CONCLUSION Mutation of c-fms codon at site 301 implied a molecular mechanism leading to hepatocellular carcinogenesis.

关 键 词：遗传学 病理学 fms基因 突变 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]