利用废水液体发酵生产单细胞蛋白的实验研究  

FERMENTATION PRODUCTION OF SING-CELL-PROTEIN (SCP) BY UTILIZATION OF WASTEWATER

作　　者：刘启富[1] 陈志谨[1] 金晓琳[1] 张克斌[1] 饶贤才[1] 胡晓梅[1] 胡福泉[1] 

机构地区：[1]第三军医大学微生物学教研室,重庆400038

出　　处：《微生物学杂志》2001年第2期8-10,共3页Journal of Microbiology

摘　　要：利用工厂废水或动物血制作培养基 ,以液体发酵方式培养产朊假丝酵母NCTC 35 76菌及甘饲 85 0 1菌。离心分离菌体 ,测定菌体生物生长量 ,并观察蔗糖、温度、酸碱度、发酵时间、种子液接种量对生物生长量的影响。生化方法分析NCTC 35 76菌单细胞蛋白的品质 ,氨基酸分析仪分析单细胞蛋白的氨基酸组成。结果表明 ,玉米淀粉厂废水 ,不必调pH ,不用添加其它任何组分 ,即可直接用于液体发酵产朊假丝酵母NCTC 35 76菌 ;pH 4.0～ 7.0均对生长量无明显影响 ;2 4h培养已达到最好生长量 ,再延长时间已不能提高产量 ;2 8℃及 37℃培养无明显差别 ,在3 %～ 17%种子液接种量范围内 ,接种量与生长量有正相关关系。研究表明 ,玉米淀粉厂废水单一成分即可用于液体发酵产朊假丝酵母NCTC 35 76菌 ,原料成本低 ,且易于产品分离纯化和工业化生产中的连续培养 ,是单细胞蛋白生产的良好途径。Wastewater from corn starch mills and animal blood were used as media without adjusting pH /and supplying any other ingredients to culture Candida utilis NCTC3576 and Gansi 8501 (a fungus strain decomposing blood) by means of submerge fermentation. The cells were collected by centrifuge. Biomass was measured by wet weighting. The effects of sucrose, temperature, pH, time, and inoculation were observed. Nutrition and amino acids of the SCP were analyzed. The results showed that pH ranged 4.0～7.0 did not affect the biomass. The SCP yields ideal level in 24h, and longer time did not increase the yield. 28℃ and 37℃ made no different for the growth. 3%～17% of inoculation was positively related to the yield. Therefore, it has a good prospect for SCP production.

关 键 词：废水利用 发酵 产朊假丝酵母 单细胞蛋白 

分 类 号：TQ926[轻工技术与工程—发酵工程] X703[环境科学与工程—环境工程]