栖土曲霉生产角蛋白酶研究  

KERATINASE PRODUCTION BY Aspergillus terricola

作　　者：曹军[1] 郝林[2] 宋志文[1] 唐晓萌[1] 张成刚[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110015 [2]沈阳师范学院生物系,辽宁沈阳110034

出　　处：《微生物学杂志》2001年第2期11-12,32,共3页Journal of Microbiology

基　　金：沈阳市青年科学基金资助项目 (19992 5 0 46 0 2 )

摘　　要：从土壤中分离出 1株可有效降解角蛋白的栖土曲霉 (Aspergillusterricola) ,经UV射线处理及发酵条件优化 ,固态发酵产酶活力提高了 80 % ,达 1980U/ g。测定了该酶的基本性质 ,其作用最适 pH和温度分别为 7.5和45℃ ,可被DTT激活 ,被甲醛、Hg2 +等抑制 ,具丝氨酸蛋白酶的特性。A keratinase producing strain was screened and identified as Aspergillus terricola. The yield of it was increased by 80% after treated with UV and optimizing the fermentation conditions. It could be activated by DTT, and inhibited by Hg+{2+} and formaldehyde etc. It has the specific property of serine protease. And its optimum pH and temperature are 7.5 and 45℃ respectively.

关 键 词：栖土曲霉 角蛋白酶 突变 优化 发酵条件 选育 

分 类 号：TQ925.2[轻工技术与工程—发酵工程] Q933[生物学—微生物学]