壳聚糖与丙烯腈接枝共聚物的制备及固定化α-淀粉酶研究  

GRAFT COPOLYMERIZATION OF AN ONTO CHITOSAN AND IMMOBILIZATION OF Α-AMYLASE ON THE GRAFT COPOLYMERS

作　　者：刘峥[1] 蒋先民[2] 

机构地区：[1]桂林工学院材料系,桂林541004 [2]广西师范大学化工系,桂林541004

出　　处：《离子交换与吸附》2001年第3期256-262,共7页Ion Exchange and Adsorption

摘　　要：本文以过硫酸钾/亚硫酸氢钠为引发体系，制备了丙烯腈接枝壳聚糖的共聚物，并以 其为载体固定化α-淀粉酶，探讨了固定化酶的最佳制备条件和固定化酶的性质，并与游离 酶、壳聚糖作为载体的固定化α-淀粉酶进行了比较，结果表明，丙烯腈接枝壳聚糖共聚物 是固定化α-淀粉酶的优良载体。Graft copolymerization of AN onto chitosan was carried out by using K2 S2O8/NaHSO3 as an initiator. Then,α-amylase was immobilized on the AN-g-chitosa n copolymer by adsorption. The effects of coupling time , pH values and the amou nt of α-amylase on immobilization and properties of the immobilized α-amylase was studied. The results show that the properties of immobilized α－amylase on the AN-g-chitosan was better than free α-amylase or the immobilized α－amylase on the chitosan.

关 键 词：壳聚糖 接枝共聚物 a-淀粉酶 固定化酶 葡萄糖 酶法生产 

分 类 号：TQ925.1[轻工技术与工程—发酵工程] Q814.2[生物学—生物工程]