金藻Isochrysis Galbana中DHA的分离与分析  

Separation and Analysis DHA from Isochrysis Galbana

作　　者：李润[1] 张万宽[1] 倪杭生[1] 戴俊彪[1] 王宏[1] 周春玉[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《应用化学》2001年第6期454-456,共3页Chinese Journal of Applied Chemistry

摘　　要：以金藻作为二十二碳六烯酸 (DHA)的新来源 ,对金藻中的脂肪酸分别用氯仿、甲醇、水 (体积比为1∶ 2∶ 0 .8)的混合液进行萃取分离和 BF3 、无水乙醇 (体积比 1∶ 3 )作酯化剂乙酯化 .用硅胶薄板检验乙酯化程度 .利用气相色谱 (GC)和气相色谱 -质谱联用仪 (GC/MS)进行定性分析 ,用内标法对 DHA进行定量分析 ,C19脂肪酸乙酯作内标 .测得藻粉中 DHA含量为 2 .2 % .Isochrysis galbana is used as a new source of DHA(docosahexaenoic acid). The fatty acid of Isochrysis galbana was extracted by a mixture of CH_3Cl, CH_3OH and H_2O(1∶2∶0.8 in volume) and esterified by a mixture of BF_3 and C_2H_5OH(1∶3 in volume), respectively. The esterification degree was detected with silica gel thin films. The quantitative analysis for DHA was carried out by GC and GC/MS techniques using ethyl ester of C_19 fatty acid as an internal standard. DHA in yield of 2.2% was obtained from algae isochrysis galbana.

关 键 词：金藻 二十二碳六烯酸 萃取 分离 分析 气相色谱 质谱 

分 类 号：Q949.262[生物学—植物学] Q946.81