茶多酚中儿茶素类的HPLC分析方法学考察  

Study on the Method of HPLC Analysis of Catechins in Tea Polyphenols

作　　者：于海宁[1] 沈生荣[1] 臧荣春[2] 冯萍[1] 

机构地区：[1]浙江大学茶学系 [2]浙江大学分析测试中心,浙江杭州310029

出　　处：《茶叶科学》2001年第1期61-64,共4页Journal of Tea Science

基　　金：浙江省科委大型仪器分析测试基金资助项目! (编号 :9912 9)

摘　　要：本文建立了一种非梯度洗脱的儿茶素HPLC定性定量分析方法 ,同时对茶多酚中的四种主要儿茶素(EC ,EGCG ,ECG ,EGC)考察了其方法学。得到最佳色谱条件为 :ODS (5 μm ,4 6× 2 5 0mm)柱 ,以重蒸水∶乙睛∶乙酸乙酯 =86∶12∶2 (v v v)为流动相 (浓硫酸调pH值至 3 0～ 4 0 ) ,流速为 1 0ml min ,UV检测波长 2 80nm。A kind of analytical method by high performance liquid chromatography(HPLC) for tea catechins with ingradient elution system was put forward In the meantime,the method was investigated in the methodological aspect through separating and determining the main four catechins (EC,ECG,EGC,EGCG) of tea polyphenols The optimum chromatographic conditions were as follows:ODS column (5 μm,4 6×250 mm),water/acetonitrile/ethylacetate (v/v/v,8 6∶12∶2) as mobile phase (add H 2SO 4 to adjust pH 3 0～4 0),UV detector (280 nm),flow rate:1 0 ml/min Moreover,the precision and stability of the method was discussed

关 键 词：儿茶素 HPLC 方法学 分析方法 茶多酚 

分 类 号：O657.7[理学—分析化学]