大豆磷脂的高效液相色谱分析  

Analysis of Soybean Phospholipids by High Performance Liquid Chromatography

作　　者：夏海涛[1] 安红[1] 刘郁芬[1] 于春玲[2] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院物理化学教研室,齐齐哈尔161006 [2]北华大学吉林林学院

出　　处：《分析化学》2001年第9期1046-1048,共3页Chinese Journal of Analytical Chemistry

摘　　要：采用正相高效液相色谱法，梯度洗脱程序和蒸发光散射检测器对大豆磷脂组成进行分析，在15 min内将大豆磷脂中4种重要组分：卵磷脂、脑磷脂、肌醇磷脂和磷脂酸与其它组分完全分离。用外标法对这4 种重要成分进行定量，线性范围为0.2～５.8 g/L；回收率为96.7～100.8%；相对标准偏差为0.82%～1.34%。方法用于实际样品测定，获得满意的结果。Soybean phospholipids were analyzed by positive-phase high performance liquid chromatography using gradient eluted program and evaporative light-scattering detector. The four important components, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidyl acid of soybean phospholipids were separated completely with other components in fifteen minutes. The quantitative analysis of the four important components has been achieved with external standard method. The linear range is 0.2 similar to 5.8 g/L. The recovery of the method is 96.7% similar to 100.8%, the relative standard deviations is 0.82% similar to 1.34%. This method has been applied to the analysis of soybean phospholipids.

关 键 词：高效液相色谱 大豆磷脂 卵磷脂 脑磷脂 分析 

分 类 号：O657.7[理学—分析化学]