DPC4基因在胰腺癌中的改变  

Alterations in DPC4 gene in pancreatic cancers

作　　者：谷丽君[1] 陈杰[1] 崔全才[1] 高洁[1] 李梅[1] 刘彤华[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院病理科,北京100730

出　　处：《中华肿瘤杂志》2001年第4期293-295,共3页Chinese Journal of Oncology

基　　金：国家杰出青年基金资助项目(39625012);国家教委跨世纪优秀人才计划基金资助项目

摘　　要：目的　研究DPC4(deletedinpancreaticcancerlocus 4)基因在胰腺癌中的改变。方法　用多聚酶链反应 单链构象多态性分析 (PCR SSCP)银染技术及PCR产物直接测序法检测胰腺癌细胞系P1、P2、P3、P4、P7,11例新鲜冷冻胰腺癌组织中DPC4基因第 1,2 ,3,4,8,11外显子的缺失和突变。结果　 5株人胰腺癌细胞系中 ,3株 (P1、P2、P3)存在DPC4基因突变 ,DPC4基因改变率为 3/ 5。 11例新鲜冷冻胰腺癌组织中 ,3例有DPC4基因的纯合性缺失 ,2例有基因突变 ,DPC4基因改变率为 5 / 11(4 5 .5 % )。结论　DPC4作为一种抑癌基因 ,其改变在胰腺癌的形成中可能起重要作用。Objective To examine the alterations in the DPC4 gene in pancreatic cancers. Methods Five pancreatic adenocarcinoma cell lines (P1, P2, P3, P4, and P7) and 11 fresh frozen pancreatic cancer tissues were monitored with polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) analysis to demonstrate the sequence of deletion and mutation in 6 exons (1, 2, 3, 4, 8, and 11) of the DPC4 gene. Results Mutation in the DPC4 gene was found in 3 of 5 cell lines (P1, P2, and P3). Three of eleven fresh frozen tissues showed homozygous deletions and two showed intragenic mutations, with a gene alteration frequency of 45.5% (5/11). Conclusion Alterations in tumor suppressor gene DPC4 may play an important role during the tumorigenesis of pancreatic cancer.

关 键 词：胰腺肿瘤 DPC4基因 纯合性缺失 基因突变 

分 类 号：R735.9[医药卫生—肿瘤]