固定化β-半乳糖苷酶催化生成低聚半乳糖  

Synthesis of Galacto-oligosaccharides with Immobilized β-galactosidase

作　　者：秦燕[1] 宁正祥[1] 胡新宇[1] 

机构地区：[1]华南理工大学食品与生物工程学院,广州510641

出　　处：《食品与发酵工业》2001年第11期12-16,共5页Food and Fermentation Industries

摘　　要：研究了聚丙烯酰胺包埋后的乳糖酶的基本酶学性质和 pH、温度、反应时间、起始乳糖质量分数和酶用量对固定化酶合成低聚糖的影响。得到填充床式连续反应优化反应条件为 4 0 %的乳糖质量分数 ,反应温度 5 5℃ ,pH 5 5 ,反应停留时间 4 5min ,酶用量 30U/g乳糖。得率可达 4 0 %galactosidase from Aspergillus oryzae entrapped in polyAcr had a better pH, temperature and holding stability The GOS was synthesized continuously in packed bed reactor The operation parameters pH, temperature, reaction time , initial lactose concentration and additive enzyme amount were optimized The maximam yilds of GOS was about 40%

关 键 词：Β-半乳糖苷酶 低聚半乳糖 固定化 合成 凝胶包埋 

分 类 号：TS201.3[轻工技术与工程—食品科学]