固体培养条件下外加营养碳源及微量元素对贝壳状革耳菌酶系产生的影响  

Effects of Supplement Carbon Sources and Trace Elements on Production of Lignocellulolytic Enzyme Profiles by Panus conchatus during the Solid State Fermentation Processes of Rice Straw

作　　者：胡平平[1] 付时雨[1] 余惠生[1] 

机构地区：[1]中国科学院广州化学研究所纤维素化学开放研究实验室,广州510650

出　　处：《纤维素科学与技术》2001年第4期44-50,共7页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金;省自然科学基金资助课题

摘　　要：研究了固体培养条件下外加营养碳源葡萄糖及土豆汁 ,微量元素Cu2 + 、Mn2 + 对贝壳状革耳菌产木素降解酶、纤维素酶和半纤维素酶的影响。结果表明 ,营养碳源和微量元素对贝壳状革耳菌漆酶的产生有显著的促进作用 ,外加碳源土豆汁要比外加葡萄糖所产生的促进作用大。对于依赖锰过氧化酶、CMC酶和半纤维素酶 ,外加营养碳源和微量元素对其产生无明显影响 ,而微晶纤维素酶在各个固体培养过程中都未检测到。The effects of supplement of nutrient carbon sources and trace elements on production of lignin-degrading enzymes, cellulolytic enzymes and xylanase were investigated in the present paper. It was found that supplement of nutrient carbon sources, either glucose or potato extractives and trace elements, either Cu 2+ or Mn 2+ could remarkably stimulate production of laccase during the solid state fermentation (SSF) by Panus conchatus . Production of laccase during SSF processes was more when potato extractives was supplemented than glucose was done. However, the effects on production of MnP were limited when either nutrient carbon sources or trace elements were supplemented. It was also found that avicelase activities were not detected during the solid-state fermentation with and without supplement of nutrient carbon sources and trace elements. The effects on production of CMCase and xylanse were also limited with and without supplement of nutrient carbon sources and trace elements.

关 键 词：贝壳状革耳菌酶系 营养碳源 微量元素 白腐菌 漆酶 纤维素酶 半纤维素酶 

分 类 号：Q55[生物学—生物化学]