微水相脂肪酶催化乙酸甲基苯甲酯立体选择性氨解反应  

LIPASE-CATALYZED ENANTIOSELECTIVE AMMONOLYSIS OF (±)-α- METHYLBENZYL ACETATE IN MICROAQUEOUS PHASE

作　　者：李慧青[1] 宗敏华[1] 何欢[1] 梁文峰[1] 

机构地区：[1]华南理工大学生物工程系,广州510640

出　　处：《微生物学通报》2001年第6期40-44,共5页Microbiology China

基　　金：广东省自然科学基金资助项目 (No . 980 5 4 3)

摘　　要：从 4种不同来源的脂肪酶 ,2种不同来源的蛋白酶中筛选出了具有较高活性和对映体选择性的能催化乙酸甲基苯甲酯氨解反应的脂肪酶Novozym 43 5。进一步探讨了氨源、酶浓度、底物浓度等因素对该酶反应的影响。结果表明 ,在优化条件下 ,氨解反应 6h ,转化率为 5 1 6 %,残留底物中 ( ) 乙酸甲基苯甲酯对映体过剩值可达 99%以上。Novozym 435 was selected from four lipases and two proteinase because of its high catalytic activity and enantiosectivity.For the ammonolysis of (±)-α-methylbenzyl acetate,The effect of ammonia sources,the concentration of enzyme and substrates on the reaction were further explored .under the optimum conditions of this study,after 6h reaction,with the enantiomeric excess of the remaining (-)-α-methylbenzyl acetate was found to be higher than 99%.

关 键 词：微水相 脂肪酶 乙酸甲基苯甲酯 立体选择性氨解反应 催化 

分 类 号：O643.3[理学—物理化学]