松针褐斑病菌的产毒培养和毒素粗提方法  

Studies on the Methods of Production and Extraction of Toxins from Lecanosticta acicola Causing Brown Spot Needle Blight of Pine

作　　者：叶建仁[1] 杨斌[1] 包宏[1] 祁高富[1] 

机构地区：[1]南京林业大学,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2001年第5期6-10,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金资助项目 (3 9870 62 7)

摘　　要：松针褐斑病菌在PD和MS培养基中生长良好 ,能产生毒素。在这两种培养基中加入敏感寄主火炬松的针叶浸汁对毒素的产生和活性没有明显影响。甲醇、乙醇、正丁醇、氯仿甲醇 (氯仿∶甲醇 =2∶3)混合溶剂等均能把毒素物质充分提取出来 ,苯、氯仿、乙酸乙酯等极性小的溶剂不能充分提取毒素。通过生测和高效液相 (HPLC)比较分析表明 ,采用MS培养液培养病菌 ,以氯仿甲醇混合溶剂提取毒素 ,将有利于毒素的产生和毒素的分离。Brown spot needle blight of pine is an important pine di sease in south China.It is caused by Lecanosticta acicola.The pathogenic fun gi could produce toxic substances in suitable media. In this study,several media were used for toxin production and some organic solvents were used to extract t oxins from the metabolites of the pathogen in MS medium.The results indicated:PD and MS culture liquids were better media for toxin production;adding water extr acting substances of lobollolly pine needles in the two media could not evidentl y affect the yields of LA-toxins;among these tested solvents,methyl,alcohol,eth anol,n-butanol and CHCl_3-MeOH mixed solvent(CHCl_3∶MeOH=2∶3)could extract LA-toxins fully;acetic ester and benzol could not extract the toxins f ully.According to the results of bioassay and HPLC the author believed using MS culture liquid to culture the pathogen and using the CHCl_3-MeOH mixed sol vent(CHCl_3∶MeOH=2∶3)to extract crude LA-toxins would be suitable to the production and seperation of LA-toxins.

关 键 词：松针褐斑病 真菌毒素 松针褐斑病菌 松树病害 产毒培养 粗提 

分 类 号：S763.712.4[农业科学—森林保护学] S763.15[农业科学—林学]