紫草抗氧化成分的提取及其活性研究  

Extraction of Antioxidant Compositions from Litospermum erythrorhizon and Study of their Antioxidant Activity

作　　者：姜爱莉[1] 孙丽芹[1] 刘玉鹏[1] 

机构地区：[1]烟台大学食品研究所,山东烟台264005

出　　处：《精细化工》2002年第1期51-54,共4页Fine Chemicals

摘　　要：分别用石油醚、三氯甲烷和乙醇依次提取紫草成分 ,用OSI仪测定了三种提取物对猪油氧化稳定性的影响 ,发现石油醚提取成分 (LE - pet)和三氯甲烷提取成分 (LE -chl)有明显的抗氧化活性 ,添加到猪油中后 ,w =0 0 2 %时 ,猪油的氧化稳定性比空白样品提高了约 1倍。对LE -pet和LE -chl进行薄层层析分离鉴定 ,得到三种化合物 :β ,β 二甲基丙烯酰紫草醌 (Ⅰ)、乙酰紫草醌 (Ⅱ)和紫草醌 (Ⅲ)。对它们的抗氧化活性进行测试 ,化合物Ⅱ和Ⅲ表现出明显的抗氧化活性 ,当w(Ⅱ)=0 .0 6 %、w(Ⅲ) =0 0 6 %时 ,猪油的氧化稳定性分别提高了 6 0 4和 3 94倍。化合物Ⅰ、Ⅱ、Ⅲ与VE均有较强的协同作用 ,化合物Ⅰ、Ⅱ、Ⅲ的质量分数为 0 .0 2 %与VE 的质量分数为 0 0 2 %共同使用时 ,其抗氧化协同系数 (SE)分别为 81 4(Ⅰ)、5 2 7(Ⅱ)和 132 6 (Ⅲ )Three kinds of extracts--LE pet,LE chl and LE eth were obtained by extracting puccoon (Litospermum erythrorhizon) with petroleum ether,chloroform and ethanol respectively.LE pet and LE chl show obvious antioxidant activity for lard by OSI (oxidation stability instrument) method. After adding LE pet or LE chl ( w =0 02%),the oxidation stability of lard was doubled compared with blank sample. LE pet and LE chl of puccoon were seperated with thin layer chromatography (TLC) and three compounds were isolated.The structures of these compounds were identified by spectroscopic methods as β,β dimethylacrylshikonin(Ⅰ), acetylshikonin(Ⅱ) and shikonin(Ⅲ).Their antioxidant properties in lard were tested with OSI.The pure compoundsⅡ and Ⅲ have obvious antioxidant activity,the oxidation stability of lard increased by 6 04(Ⅱ) and 3 94(Ⅲ) times when w (Ⅱ or Ⅲ)=0 06%.Compound Ⅰ,Ⅱ and Ⅲ all show obvious synergistic effects with VE.When adding Ⅰ,Ⅱ and Ⅲ( w =0 02%) with VE( w =0 02%),the synergistic coefficients are 81 4(Ⅰ),52 7(Ⅱ) and 132 6(Ⅲ) respectively.

关 键 词：紫草 抗氧化活性 β β-二甲基丙烯酰紫草醌 乙酰紫草醌 紫草醌 活性 提取 中药 

分 类 号：R284.2[医药卫生—中药学] R282.71[医药卫生—中医学]