法夫酵母响应pH-stat的底物流加促进虾青素合成的研究  

High cell density fermentation of Xanthophyllomyces dendrorhous and its overproducing of astaxanthin by means of ph -stat with glucose and ammonia fed batch

作　　者：梁新乐[1] 岑沛霖[2] 励建荣[1] 张虹[1] 张大中 俞黎南 

机构地区：[1]杭州商学院生物工程系,杭州310035 [2]浙江大学生物化工研究所,杭州310027

出　　处：《上海交通大学学报（农业科学版）》2001年第2期87-90,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：浙江省高教委青年基金资助项目 (纵 99 3 1)

摘　　要：本文主要研究了采用响应 pH -stat的流加培养模式 ,进行Xanthophyllomycesdendrorhous高细胞密度培养及虾青素合成。在该补料间歇培养模式中 ,发酵过程 pH的恒定控制采用 2 .78mol L葡萄糖及 3mol L氨水来控制 ,同时起到流加葡萄糖的作用。试验结果表明 ,虾青素积累量于 78h可达 4.0 5mg L ,菌体干重在第 84h获最大值 14.6 2g L。与不流加葡萄糖、氨水的间歇培养相比 ,虾青素含量和菌体干重分别增加 2 .89、5 .2To examine the new approach for high cell density fermentation and high production of astaxanthin in the red yeast of Xanthophyllomyces dendrorhous , fed batch with pH stat controlled by 3mol/L ammonia and 2.78 mol/L glucose was performed, where pH change were responded by glucose and ammonia, not sulfuric acid and sodium hydrate. The experiment results showed that DCW (dry cell weight) was high by 14.62g/L at 84h, and astaxanthin volumetric yield was up to 4.05mg/L at 78h. Compared with the batch fermentation without pH stat, the productivity of astaxanthin and DCW were improved by 2.89 and 5.22 times respectively.

关 键 词：Xanthophyllomyces dendrorhous 虾青素 高密度培养 流加培养 法夫酵母 

分 类 号：TQ611[化学工程—精细化工] TQ929[轻工技术与工程—发酵工程]