温度诱导双水相分离纯化细菌素工艺条件的选取与优化  

Optimizing Conditions for Bacteriocin Isolation and Purification in Temperature-induced Aqueous Two-phase Systems

作　　者：李孱[1,2] 蔡昭铃[1] 丛威[1] 白景华[3] 欧阳藩[1] 

机构地区：[1]中国科学院化工冶金研究所生化工程国家重点实验室 [2]湖北大学生命科学院,湖北武汉430062 [3]湖北大学生命科学院

出　　处：《过程工程学报》2001年第4期412-415,共4页The Chinese Journal of Process Engineering

摘　　要：利用TritonX-114可萃取疏水性蛋白质的性质,研究了温度诱导双水相分离纯化细菌素乳链菌肽, 用响应面法对成相成份TritonX-114和离子强度进行了优化,最佳浓度分别为3.27% TritonX-114,0.85 mol/L氯化钠. 优化过程中,pH固定为2.0,温度固定为30C, 在此条件下乳链菌肽的萃取率为92%,纯化系数为9.Bacteriocin nisin was isolated and purified in temperature-induced aqueous two-phase systems using the capacity of extracting hydrophobic proteins of TritonX-114. The concentrations of sodium chloride and TritonX-114which can form the aqueous two-phase systems were optimized by the response surface method. The full-factorial design was used to assess the main effects of factors and determine the path of steepest ascent to the optimal region of the aqueous two-phase system composition. The optimum concentrations of TritonX-114 and sodium chloride were then easily determined by using a central composite design and were found to be 3.27% of TritonX-114 and 0.85 mol/L sodium chloride. The recovery and purification factor were 92% and 9.05 respectively under the optimum conditions.

关 键 词：温度诱导双水相系统 萃取 细菌素 响应面法 优化 食品添加剂 

分 类 号：TS202.3[轻工技术与工程—食品科学] Q936[轻工技术与工程—食品科学与工程]