黄海黄杆菌YS-9412-130低温碱性蛋白酶发酵过程动力学研究  

Kinetic model of low-temperatue alkaline protease fermentation process from Flavobacterium YS-9412-130

作　　者：孙谧[1] 王跃军[1] 李民[2] 张云波[1] 洪义国[1] 阎晓玲[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所海洋酶与酶工程开放实验室,青岛266071 [2]中国科学院生物工程中心,上海200233

出　　处：《海洋水产研究》2001年第3期14-23,共10页Marine Fisheries Research

基　　金：国家"海洋 86 3"项目 ( 86 3-819-0 6 -0 1)资助

摘　　要：应用自动控制发酵设备 ,对黄海黄杆菌 YS- 94 12 - 130碱性蛋白酶发酵动力学和工艺条件进行了研究。从基本的动力学概念和方程出发 ,通过分批发酵试验摸索了黄海黄杆菌 YS- 94 12 - 130生长与代谢的基本规律 ,证明了发酵过程中低温碱性蛋白酶的形成为生长部分关联型。采用补料分批培养方法限制生长基质浓度 ,确定其一系列参数值 。With autocontrol fermentation equipment, the kinetic model and the technology of low temperature alkaline protease fermentation and metabolism of Flavobacterium YS 9412 130 were studied. Based on kinetic concept and equation,the basic law of the growth and metabolism of Flavobacterium YS 9412 130 was researched with batch fermentation test.The data showed that the fermentation type was one partially related to the growth. A series of parameter values were obtained by controlling the concentration of the growth limiting substrate with the fed batch culture.Thereby, the kinetic mathematical model was derived to describe the relationship between cell growth rate and product synthesis rate.

关 键 词：黄海黄杆菌 YS-9412-130 海洋低温碱性蛋白酶 发酵动力学 补料分批培养 

分 类 号：TQ925[轻工技术与工程—发酵工程]