原生质体融合和秋水仙素染色体加倍构建强发酵淀粉的糖化酵母研究  

Study on potent starch-fermenting strains of Saccharomyces diastaticus constructed by protoplast fusion and colchicine chromosome treatment

作　　者：李春丽[1] 金国英[2] 李桃生[3] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]杭州师范学院生命科学学院,浙江杭州310036 [3]浙江大学生命科学学院,浙江杭州310012

出　　处：《河南农业大学学报》2002年第1期1-6,共6页Journal of Henan Agricultural University

基　　金：国家自然科学基金资助项目 (3 92 70 3 95 )

摘　　要：通过原生质体融合或秋水仙素染色体加倍技术 ,构建成交配型位点基因纯合 (α/α)的二倍体 .对所获二倍体及其亲株的糖化酶酶活性进行测定 .结果表明 ,这 3个非连锁的糖化酶等效异位基因表现出显著的剂量效应 ,应用中 3株纯合二倍体CIY187 14 (STA3/STA3) ,SFY5 5 (STA2 /STA2 )和CI12C 6 6 (STA1/STA1)菌株 ,其酶活性分别是其亲株的 2 4 4 ,2 31和 2 31倍 。Diploid strains homozygous for both MTA and STA genes and Diploid strains homozygous for MTA genes but inter combinative for STA genes were constructed by means of the protoplast fusion and colchicine chromosome treatment The results of glucoamylase activity determination of the diploids obtained and their parents indicated that the gene dosage effect of these three unlinked polymeric genes coded glucoamylase is obvious.In practice the glucoamylase activity of three homozygons diploid strains CIY187 14(α/α,STA3/STA3),SFY5 5(α/α,STA2/STA2) and CI12C 66(α/α,STA1/STA1)were respectively 2 44,2 31 and 2 31 fold as compared with that of their haploid parent strains These experimental results also suggested that the new strain Saccharomyces diastaticus with high glucoamylase activity could be constructed using dosage effect of the glucoamylase genes

关 键 词：糖化酶基因 糖化酵母 基因剂量效应 原生质体融合 秋水仙素 染色体加倍技术 二倍体 发酵淀粉 

分 类 号：TQ925[轻工技术与工程—发酵工程] TQ926