GABA_A Receptor Activity Suppresses the Transition from Interictal to Ictal Epileptiform Discharges in Juvenile Mouse Hippocampus  

作　　者：Yan-Yan Chang Xin-Wei Gong Hai-Qing Gong Pei-Ji Liang Pu-Ming Zhang Qin-Chi Lu 

机构地区：[1]Department of Neurology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University [2]School of Biomedical Engineering, Shanghai Jiao Tong University

出　　处：《Neuroscience Bulletin》2018年第6期1007-1016,共10页神经科学通报（英文版）

基　　金：supported by the Key Basic Research Project of Science and Technology Commission of Shanghai (13DJ1400303);the Shanghai Jiao Tong University Fund for Interdisciplinary Research for Medical Applications (YG2012ZD08);the Seed Fund of Ren Ji Hospital (RJ ZZ13-005)

摘　　要：Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment.We used Mg2+-free artificial cerebrospinal fluid(ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg2+-free ACSF for 10 min–20 min, synchronous recurrent seizurelike events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges(IIDs) to pre-ictal epileptiform discharges(PIDs), and then to IDs.During the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 lmol/L of the GABAAreceptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 lmol/L muscimol abolished all the epileptiform discharges. When the GABAAreceptor antagonist bicuculline was applied at 10 lmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABAAreceptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment.We used Mg^(2+)-free artificial cerebrospinal fluid(ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg^(2+)-free ACSF for 10 min–20 min, synchronous recurrent seizurelike events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges(IIDs) to pre-ictal epileptiform discharges(PIDs), and then to IDs.During the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 lmol/L of the GABAAreceptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 lmol/L muscimol abolished all the epileptiform discharges. When the GABAAreceptor antagonist bicuculline was applied at 10 lmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABAAreceptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.

关 键 词：Epileptiform discharge Gamma-aminobutyric acid BICUCULLINE MUSCIMOL Micro-electrode array Hippocampal slice 

分 类 号：R[医药卫生]